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http://purl.uniprot.org/citations/19877527http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19877527http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To study the influence of atRA on iron metabolism in cultured primary rat hepatocyte.

Methods

Rat primary hepatocytes were isolated by two-step in situ collagenase perfusion method by Seglen, and after that Cell viability was observed by 0.4% trypan blue. And then primary hepatocyte were treated into 6 wells plate with 0, 0.5, 1 and 50 micromol/L atRA and DMEM contained 10% fetal bovine serum. After 72h, IRP2 mRNA, TFR mRNA, Fnm RNA levels were measured by RT-PCR.

Results

VA deficiency can decrease the viability and function. Moreover hepatic IRP2 mRNA and TFRmRNA levels were increased by VA deficiency, which diminishing expression of Fn mRNA.

Conclusion

vitamin A deficiency can change cellular iron metabolism by inducing IRP2-Fn-TFR pathway. AtRA supplementation inhibited the increase in IRP2 mRNA expression. Taken together, these results indicate that vitamin A deficiency can regulate iron metabolism by IRP2-TFR-Fn pathway."xsd:string
http://purl.uniprot.org/citations/19877527http://purl.uniprot.org/core/author"Liu B."xsd:string
http://purl.uniprot.org/citations/19877527http://purl.uniprot.org/core/author"Jiang S."xsd:string
http://purl.uniprot.org/citations/19877527http://purl.uniprot.org/core/author"Lan L."xsd:string
http://purl.uniprot.org/citations/19877527http://purl.uniprot.org/core/author"Shi R."xsd:string
http://purl.uniprot.org/citations/19877527http://purl.uniprot.org/core/author"Wang C."xsd:string
http://purl.uniprot.org/citations/19877527http://purl.uniprot.org/core/author"Wang K."xsd:string
http://purl.uniprot.org/citations/19877527http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/19877527http://purl.uniprot.org/core/name"Wei Sheng Yan Jiu"xsd:string
http://purl.uniprot.org/citations/19877527http://purl.uniprot.org/core/pages"603-606"xsd:string
http://purl.uniprot.org/citations/19877527http://purl.uniprot.org/core/title"[Effect of all-trans retinoic acid deficiency on iron metabolism in rat primary hepatocyte]."xsd:string
http://purl.uniprot.org/citations/19877527http://purl.uniprot.org/core/volume"38"xsd:string
http://purl.uniprot.org/citations/19877527http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/19877527
http://purl.uniprot.org/citations/19877527http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/19877527
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