| http://purl.uniprot.org/citations/19883205 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19883205 | http://www.w3.org/2000/01/rdf-schema#comment | "In this study we examined whether the levels of gene expressions of the three beta-adrenergic receptor (betaAR) subtypes, beta(1), beta(2), and beta(3), contribute to age-related increase in betaAR density. Liver membranes and total RNA were prepared from young (4-to 6-month-old) and old (24-month-old) male Fischer 344 rats. betaAR density (B(max)) in liver membranes was measured by a radioligand receptor binding assay using the receptor subtype nonselective betaAR antagonist (125)I-pindolol as the radioligand. Steady-state levels of beta(2)AR mRNA in rat liver were measured by Northern blot analysis; because of the low abundance of beta(1)AR and beta(3)AR mRNA in rat liver, the expressions of these genes were measured by a semiquantitative RT-PCR or an RT-PCR. Scatchard analysis of saturation binding curves of the binding assay confirmed an age-related increase in B(max) (young: 7.1 +/- 0.8 fmol/mg protein vs. old: 18.1 +/-4.3 fmol/mg protein). No age-related differences were found in the levels of beta(2)AR mRNA. However, semiquantitative RT-PCR revealed an approximately twofold increase in beta(1)AR mRNA level between young and old rats (P < 0.05). beta(1)AR mRNA levels were also correlated with B(max) values for (125)I-pindolol binding sites in individual rats (r = 0.67; P = 0.012). beta(3)AR mRNA, which was demonstrable in rat white adipose tissue by RT-PCR, was generally not detected in livers from young or old rats, with the exception of two old rats with the highest B(max). These results suggest that an age-related increase of beta(1)AR gene expression contributes to increased betaAR density and beta adrenergic responsiveness in rat liver during aging."xsd:string |
| http://purl.uniprot.org/citations/19883205 | http://purl.org/dc/terms/identifier | "doi:10.3109/10799890903358206"xsd:string |
| http://purl.uniprot.org/citations/19883205 | http://purl.uniprot.org/core/author | "Jin W."xsd:string |
| http://purl.uniprot.org/citations/19883205 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/19883205 | http://purl.uniprot.org/core/name | "J Recept Signal Transduct Res"xsd:string |
| http://purl.uniprot.org/citations/19883205 | http://purl.uniprot.org/core/pages | "24-30"xsd:string |
| http://purl.uniprot.org/citations/19883205 | http://purl.uniprot.org/core/title | "Age-related increase of beta1-adrenergic receptor gene expression in rat liver: a potential mechanism contributing to increased beta-adrenergic receptor density and responsiveness during aging."xsd:string |
| http://purl.uniprot.org/citations/19883205 | http://purl.uniprot.org/core/volume | "30"xsd:string |
| http://purl.uniprot.org/citations/19883205 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19883205 |
| http://purl.uniprot.org/citations/19883205 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19883205 |
| http://purl.uniprot.org/uniprot/#_A0A0M4LAM5-mappedCitation-19883205 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19883205 |
| http://purl.uniprot.org/uniprot/#_A0A0G2JSP7-mappedCitation-19883205 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19883205 |
| http://purl.uniprot.org/uniprot/#_P18090-mappedCitation-19883205 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19883205 |
| http://purl.uniprot.org/uniprot/A0A0G2JSP7 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19883205 |
| http://purl.uniprot.org/uniprot/P18090 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19883205 |
| http://purl.uniprot.org/uniprot/A0A0M4LAM5 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19883205 |