| http://purl.uniprot.org/citations/19893607 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19893607 | http://www.w3.org/2000/01/rdf-schema#comment | "The removal of introns from eukaryotic RNA transcripts requires the activities of five multi-component ribonucleoprotein complexes and numerous associated proteins. The lack of mutations affecting splicing factors essential for animal survival has limited the study of the in vivo regulation of splicing. From a screen for suppressors of the Caenorhabditis elegans unc-93(e1500) rubberband Unc phenotype, we identified mutations in genes that encode the C. elegans orthologs of two splicing factors, the U2AF large subunit (UAF-1) and SF1/BBP (SFA-1). The uaf-1(n4588) mutation resulted in temperature-sensitive lethality and caused the unc-93 RNA transcript to be spliced using a cryptic 3' splice site generated by the unc-93(e1500) missense mutation. The sfa-1(n4562) mutation did not cause the utilization of this cryptic 3' splice site. We isolated four uaf-1(n4588) intragenic suppressors that restored the viability of uaf-1 mutants at 25 degrees C. These suppressors differentially affected the recognition of the cryptic 3' splice site and implicated a small region of UAF-1 between the U2AF small subunit-interaction domain and the first RNA recognition motif in affecting the choice of 3' splice site. We constructed a reporter for unc-93 splicing and using site-directed mutagenesis found that the position of the cryptic splice site affects its recognition. We also identified nucleotides of the endogenous 3' splice site important for recognition by wild-type UAF-1. Our genetic and molecular analyses suggested that the phenotypic suppression of the unc-93(e1500) Unc phenotype by uaf-1(n4588) and sfa-1(n4562) was likely caused by altered splicing of an unknown gene. Our observations provide in vivo evidence that UAF-1 can act in regulating 3' splice-site choice and establish a system that can be used to investigate the in vivo regulation of RNA splicing in C. elegans."xsd:string |
| http://purl.uniprot.org/citations/19893607 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pgen.1000708"xsd:string |
| http://purl.uniprot.org/citations/19893607 | http://purl.uniprot.org/core/author | "Horvitz H.R."xsd:string |
| http://purl.uniprot.org/citations/19893607 | http://purl.uniprot.org/core/author | "Ma L."xsd:string |
| http://purl.uniprot.org/citations/19893607 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19893607 | http://purl.uniprot.org/core/name | "PLoS Genet"xsd:string |
| http://purl.uniprot.org/citations/19893607 | http://purl.uniprot.org/core/pages | "e1000708"xsd:string |
| http://purl.uniprot.org/citations/19893607 | http://purl.uniprot.org/core/title | "Mutations in the Caenorhabditis elegans U2AF large subunit UAF-1 alter the choice of a 3' splice site in vivo."xsd:string |
| http://purl.uniprot.org/citations/19893607 | http://purl.uniprot.org/core/volume | "5"xsd:string |
| http://purl.uniprot.org/citations/19893607 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19893607 |
| http://purl.uniprot.org/citations/19893607 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19893607 |
| http://purl.uniprot.org/uniprot/P90978#attribution-02E8FD1D7BCF7A443717E13C3BC3BF2F | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/19893607 |
| http://purl.uniprot.org/uniprot/#_G5EF97-mappedCitation-19893607 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19893607 |
| http://purl.uniprot.org/uniprot/#_P90978-mappedCitation-19893607 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19893607 |
| http://purl.uniprot.org/uniprot/#_Q93380-mappedCitation-19893607 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19893607 |
| http://purl.uniprot.org/uniprot/G5EF97 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19893607 |
| http://purl.uniprot.org/uniprot/P90978 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19893607 |
| http://purl.uniprot.org/uniprot/Q93380 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19893607 |