| http://purl.uniprot.org/citations/19893608 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19893608 | http://www.w3.org/2000/01/rdf-schema#comment | "The human testis has almost as high a frequency of alternative splicing events as brain. While not as extensively studied as brain, a few candidate testis-specific splicing regulator proteins have been identified, including the nuclear RNA binding proteins RBMY and hnRNP G-T, which are germ cell-specific versions of the somatically expressed hnRNP G protein and are highly conserved in mammals. The splicing activator protein Tra2beta is also highly expressed in the testis and physically interacts with these hnRNP G family proteins. In this study, we identified a novel testis-specific cassette exon TLE4-T within intron 6 of the human transducing-like enhancer of split 4 (TLE4) gene which makes a more transcriptionally repressive TLE4 protein isoform. TLE4-T splicing is normally repressed in somatic cells because of a weak 5' splice site and surrounding splicing-repressive intronic regions. TLE4-T RNA pulls down Tra2beta and hnRNP G proteins which activate its inclusion. The germ cell-specific RBMY and hnRNP G-T proteins were more efficient in stimulating TLE4-T incorporation than somatically expressed hnRNP G protein. Tra2b bound moderately to TLE4-T RNA, but more strongly to upstream sites to potently activate an alternative 3' splice site normally weakly selected in the testis. Co-expression of Tra2beta with either hnRNP G-T or RBMY re-established the normal testis physiological splicing pattern of this exon. Although they can directly bind pre-mRNA sequences around the TLE4-T exon, RBMY and hnRNP G-T function as efficient germ cell-specific splicing co-activators of TLE4-T. Our study indicates a delicate balance between the activity of positive and negative splicing regulators combinatorially controls physiological splicing inclusion of exon TLE4-T and leads to modulation of signalling pathways in the testis. In addition, we identified a high-affinity binding site for hnRNP G-T protein, showing it is also a sequence-specific RNA binding protein."xsd:string |
| http://purl.uniprot.org/citations/19893608 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pgen.1000707"xsd:string |
| http://purl.uniprot.org/citations/19893608 | http://purl.uniprot.org/core/author | "Liu Y."xsd:string |
| http://purl.uniprot.org/citations/19893608 | http://purl.uniprot.org/core/author | "Pang S."xsd:string |
| http://purl.uniprot.org/citations/19893608 | http://purl.uniprot.org/core/author | "Sun Y.H."xsd:string |
| http://purl.uniprot.org/citations/19893608 | http://purl.uniprot.org/core/author | "Kudla M."xsd:string |
| http://purl.uniprot.org/citations/19893608 | http://purl.uniprot.org/core/author | "Elliott D.J."xsd:string |
| http://purl.uniprot.org/citations/19893608 | http://purl.uniprot.org/core/author | "Bourgeois C.F."xsd:string |
| http://purl.uniprot.org/citations/19893608 | http://purl.uniprot.org/core/author | "Stevenin J."xsd:string |
| http://purl.uniprot.org/citations/19893608 | http://purl.uniprot.org/core/author | "Dreumont N."xsd:string |
| http://purl.uniprot.org/citations/19893608 | http://purl.uniprot.org/core/author | "Kister L."xsd:string |
| http://purl.uniprot.org/citations/19893608 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19893608 | http://purl.uniprot.org/core/name | "PLoS Genet"xsd:string |
| http://purl.uniprot.org/citations/19893608 | http://purl.uniprot.org/core/pages | "e1000707"xsd:string |
| http://purl.uniprot.org/citations/19893608 | http://purl.uniprot.org/core/title | "The germ cell nuclear proteins hnRNP G-T and RBMY activate a testis-specific exon."xsd:string |
| http://purl.uniprot.org/citations/19893608 | http://purl.uniprot.org/core/volume | "5"xsd:string |
| http://purl.uniprot.org/citations/19893608 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19893608 |
| http://purl.uniprot.org/citations/19893608 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19893608 |
| http://purl.uniprot.org/uniprot/#_A0A0A0MT79-mappedCitation-19893608 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19893608 |
| http://purl.uniprot.org/uniprot/#_B2RUT9-mappedCitation-19893608 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19893608 |
| http://purl.uniprot.org/uniprot/#_A8K8A6-mappedCitation-19893608 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19893608 |
| http://purl.uniprot.org/uniprot/#_A6NIY7-mappedCitation-19893608 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19893608 |
| http://purl.uniprot.org/uniprot/#_B3KQ29-mappedCitation-19893608 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19893608 |
| http://purl.uniprot.org/uniprot/#_Q04727-mappedCitation-19893608 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19893608 |