Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/19906316http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19906316http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19906316http://www.w3.org/2000/01/rdf-schema#comment"

Background

Alternative polyadenylation sites within a gene can lead to alternative transcript variants. Although bioinformatic analysis has been conducted to detect polyadenylation sites using nucleic acid sequences (EST/mRNA) in the public databases, one special type, single-block EST is much less emphasized. This bias leaves a large space to discover novel transcript variants.

Results

In the present study, we identified novel transcript variants in the human genome by detecting intronic polyadenylation sites. Poly(A/T)-tailed ESTs were obtained from single-block ESTs and clustered into 10,844 groups standing for 5,670 genes. Most sites were not found in other alternative splicing databases. To verify that these sites are from expressed transcripts, we analyzed the supporting EST number of each site, blasted representative ESTs against known mRNA sequences, traced terminal sequences from cDNA clones, and compared with the data of Affymetrix tiling array. These analyses confirmed about 84% (9,118/10,844) of the novel alternative transcripts, especially, 33% (3,575/10,844) of the transcripts from 2,704 genes were taken as high-reliability. Additionally, RT-PCR confirmed 38% (10/26) of predicted novel transcript variants.

Conclusion

Our results provide evidence for novel transcript variants with intronic poly(A) sites. The expression of these novel variants was confirmed with computational and experimental tools. Our data provide a genome-wide resource for identification of novel human transcript variants with intronic polyadenylation sites, and offer a new view into the mystery of the human transcriptome."xsd:string
http://purl.uniprot.org/citations/19906316http://purl.org/dc/terms/identifier"doi:10.1186/1471-2164-10-518"xsd:string
http://purl.uniprot.org/citations/19906316http://purl.org/dc/terms/identifier"doi:10.1186/1471-2164-10-518"xsd:string
http://purl.uniprot.org/citations/19906316http://purl.uniprot.org/core/author"Ma D."xsd:string
http://purl.uniprot.org/citations/19906316http://purl.uniprot.org/core/author"Ma D."xsd:string
http://purl.uniprot.org/citations/19906316http://purl.uniprot.org/core/author"Wang P."xsd:string
http://purl.uniprot.org/citations/19906316http://purl.uniprot.org/core/author"Wang P."xsd:string
http://purl.uniprot.org/citations/19906316http://purl.uniprot.org/core/author"Yu P."xsd:string
http://purl.uniprot.org/citations/19906316http://purl.uniprot.org/core/author"Yu P."xsd:string
http://purl.uniprot.org/citations/19906316http://purl.uniprot.org/core/author"Gao P."xsd:string
http://purl.uniprot.org/citations/19906316http://purl.uniprot.org/core/author"Gao P."xsd:string
http://purl.uniprot.org/citations/19906316http://purl.uniprot.org/core/author"Shi T."xsd:string
http://purl.uniprot.org/citations/19906316http://purl.uniprot.org/core/author"Shi T."xsd:string
http://purl.uniprot.org/citations/19906316http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/19906316http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/19906316http://purl.uniprot.org/core/name"BMC Genomics"xsd:string
http://purl.uniprot.org/citations/19906316http://purl.uniprot.org/core/name"BMC Genomics"xsd:string
http://purl.uniprot.org/citations/19906316http://purl.uniprot.org/core/pages"518"xsd:string
http://purl.uniprot.org/citations/19906316http://purl.uniprot.org/core/pages"518"xsd:string
http://purl.uniprot.org/citations/19906316http://purl.uniprot.org/core/title"Discovery of novel human transcript variants by analysis of intronic single-block EST with polyadenylation site."xsd:string
http://purl.uniprot.org/citations/19906316http://purl.uniprot.org/core/title"Discovery of novel human transcript variants by analysis of intronic single-block EST with polyadenylation site."xsd:string
http://purl.uniprot.org/citations/19906316http://purl.uniprot.org/core/volume"10"xsd:string
http://purl.uniprot.org/citations/19906316http://purl.uniprot.org/core/volume"10"xsd:string
▶