| http://purl.uniprot.org/citations/19908067 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19908067 | http://www.w3.org/2000/01/rdf-schema#comment | "PurposeThe molecular mechanisms and candidate genes involved in development of meningiomas still needs investigation and elucidation.MethodsIn the present study 60 meningiomas were analyzed regarding changes of tumor suppressor gene E-cadherin (CDH1), a component of adherens junction and an indirect modulator of the wnt signaling. Gene instability was tested by polymerase chain reaction/loss of heterozygosity (LOH) method. Protein expression was analyzed by immunohistochemistry.ResultsThe results of our analysis showed altogether 32% of samples with LOH of the CDH1 gene. Interestingly, another type of genomic instability was detected; replication error-positive samples (RER+). Three out of 28 heterozygous samples were RER+ (11%). The instability is the result of impaired cellular mismatch repair. Fibrous and angiomatous cases showed higher percent of genetic changes, 67 and 75%, respectively. Immunostaining showed that overall 73% of samples had downregulation of E-cadherin expression. Intense downregulation of E-cadherin was noticed in tumors with grades II and III. Five out of nine samples with LOH were accompanied with the downregulation of E-cadherin protein expression (56%). One RER+ sample had lower expression of E-cadherin. We noticed that 36.4% of samples with lower E-cadherin expression had beta-catenin located in the nucleus. Also, 75% of samples with genomic instabilities had beta-catenin in the nucleus. Our findings demonstrated that there is significant association between the genetic changes of CDH1 and the nuclear localization of beta-catenin protein (chi(2) = 5.25, df = 1, P < 0.022). Beta-catenin was progressively upregulated from meningothelial to atypical, while 60% of anaplastic showed upregulation and nuclear localization of the protein.ConclusionsOur results suggest that genetic instabilities of the E-cadherin gene have a role in meningioma development and progression. Detected microsatellite instability indicates that mismatch repair may also be targeted in meningioma."xsd:string |
| http://purl.uniprot.org/citations/19908067 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00432-009-0708-z"xsd:string |
| http://purl.uniprot.org/citations/19908067 | http://purl.uniprot.org/core/author | "Hrascan R."xsd:string |
| http://purl.uniprot.org/citations/19908067 | http://purl.uniprot.org/core/author | "Musani V."xsd:string |
| http://purl.uniprot.org/citations/19908067 | http://purl.uniprot.org/core/author | "Tomas D."xsd:string |
| http://purl.uniprot.org/citations/19908067 | http://purl.uniprot.org/core/author | "Pecina-Slaus N."xsd:string |
| http://purl.uniprot.org/citations/19908067 | http://purl.uniprot.org/core/author | "Nikuseva Martic T."xsd:string |
| http://purl.uniprot.org/citations/19908067 | http://purl.uniprot.org/core/author | "Zeljko M."xsd:string |
| http://purl.uniprot.org/citations/19908067 | http://purl.uniprot.org/core/author | "Deak A.J."xsd:string |
| http://purl.uniprot.org/citations/19908067 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/19908067 | http://purl.uniprot.org/core/name | "J Cancer Res Clin Oncol"xsd:string |
| http://purl.uniprot.org/citations/19908067 | http://purl.uniprot.org/core/pages | "695-702"xsd:string |
| http://purl.uniprot.org/citations/19908067 | http://purl.uniprot.org/core/title | "Genetic and protein changes of E-cadherin in meningiomas."xsd:string |
| http://purl.uniprot.org/citations/19908067 | http://purl.uniprot.org/core/volume | "136"xsd:string |
| http://purl.uniprot.org/citations/19908067 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19908067 |
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