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http://purl.uniprot.org/citations/19954024http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19954024http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To investigate whether down-regulation of Twist1 could change sensitivity of nasopharyngeal carcinoma cell line HNE1 to taxol.

Methods

HNE1 cells were transfected with the small interfering RNA (siRNA) expression vector pSuppressor-Retro-Si-Twist, containing the short hairpin RNA (shRNA) sequence targeting the Twist gene-coding region by Fugene 6. Positive clones were then selected in Neomycin (400 microg/ml) for 21 days. The low expressions of Twist1 were examined by real-time reverse-transcription polymerase chain reaction (RT-PCR) and Western blot. Drug sensitivity of si-Twist1 HNE1 to taxol was determined by Annexin V-fluorescein isothiocyanate( FITC)/propidium lodide (PI) double-labeled flow cytometry and detection of DNA ladder. The Effect of Twist1 inactivation on HNE1 cell proliferation was observed by MTT assay and flow cytometry.

Results

Annexin V-FITC-PI assay showed that apoptosis ratio was 40.2% in si-Twist HNE1 after treated with 10 ng/ml taxol, significantly higher than that in the control siRNA group 24.3%. The deference had statistic meaning. After the re-expression of HNE1, apoptosis ratio was 44.80% +/- 4.80% (x +/-s) in low Twist1 protein expression group and that was 27.00% +/-2.91% in high expression group. The deference had statistic meaning (t = 4.374, P = 0.049). Real time PCR test revealed apoptosis protein bcl-2 expression in si-Twist HNE1 was 0.28 +/-0.05, significantly lower than that in the control siRNA HNE1 (0.57 +/-0.08, t = 6.710, P = 0.021), nevertheless, significant bax and bcl-XL changes were not observed (t = 2.000, P = 0.184 and t = 1.502, P = 0.272). MTT and FCM showed that down-regulation of Twist1 did not alter cell proliferation rate (P>0.05).

Conclusions

Down-regulation of Twist1 could increase drug sensitivity of nasopharyngeal carcinoma cell line HNE1 to taxol by inducing apoptosis. These results suggested that Twist1 may be a promising treatment target for nasopharyngeal carcinoma therapy."xsd:string
http://purl.uniprot.org/citations/19954024http://purl.uniprot.org/core/author"Li Z."xsd:string
http://purl.uniprot.org/citations/19954024http://purl.uniprot.org/core/author"Li D."xsd:string
http://purl.uniprot.org/citations/19954024http://purl.uniprot.org/core/author"Li S.J."xsd:string
http://purl.uniprot.org/citations/19954024http://purl.uniprot.org/core/author"Ma Y.P."xsd:string
http://purl.uniprot.org/citations/19954024http://purl.uniprot.org/core/author"Bao J.M."xsd:string
http://purl.uniprot.org/citations/19954024http://purl.uniprot.org/core/author"Meng D.W."xsd:string
http://purl.uniprot.org/citations/19954024http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/19954024http://purl.uniprot.org/core/name"Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi"xsd:string
http://purl.uniprot.org/citations/19954024http://purl.uniprot.org/core/pages"504-508"xsd:string
http://purl.uniprot.org/citations/19954024http://purl.uniprot.org/core/title"[Down-regulation of Twist1 increases the sensitivity of nasopharyngeal carcinoma cell lines HNE1 to taxol]."xsd:string
http://purl.uniprot.org/citations/19954024http://purl.uniprot.org/core/volume"44"xsd:string
http://purl.uniprot.org/citations/19954024http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/19954024
http://purl.uniprot.org/citations/19954024http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/19954024
http://purl.uniprot.org/uniprot/#_Q15672-mappedCitation-19954024http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19954024
http://purl.uniprot.org/uniprot/Q15672http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/19954024