http://purl.uniprot.org/citations/19962718 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/19962718 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/19962718 | http://www.w3.org/2000/01/rdf-schema#comment | "gamma-Conglutin, a glycoprotein from Lupinus albus seed, has been characterized at molecular level but its physiological function is still unknown. gamma-Conglutin shares a high structural similarity with xyloglucan-specific endo-beta-1,4-glucanase inhibitor proteins (XEGIPs) and Triticum aestivum xylanase inhibitor (TAXI-I), which act specifically against fungal glycosyl hydrolase belonging to families 12 and 11, respectively. To assess the possible involvement of gamma-conglutin in plant defense, germinating lupin seeds were incubated with chitosan. The relative quantification of gamma-conglutin mRNA extracted from cotyledons was then carried out by RT-qPCR and indicated that chitosan strongly elicited the expression of gamma-conglutin. Moreover, biochemical trials aimed to test the inhibitory capacity of the protein have been also carried out. gamma-Conglutin failed to inhibit representative fungal endo-glucanases and other cell wall-degrading enzymes. To explain the lack of inhibitory capacity we investigated the possible structural differences between gamma-conglutin and XEGIPs and TAXI-I, including the construction of a predictive 3D model of the protein. Bioinformatic analysis suggests that the lack of inhibitory activity of gamma-conglutin can be attributed to sequence differences in the inhibitor interaction domains, and in particular to a sequence deletion in one of the functional loops."xsd:string |
http://purl.uniprot.org/citations/19962718 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.phytochem.2009.11.001"xsd:string |
http://purl.uniprot.org/citations/19962718 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.phytochem.2009.11.001"xsd:string |
http://purl.uniprot.org/citations/19962718 | http://purl.uniprot.org/core/author | "Duranti M."xsd:string |
http://purl.uniprot.org/citations/19962718 | http://purl.uniprot.org/core/author | "Duranti M."xsd:string |
http://purl.uniprot.org/citations/19962718 | http://purl.uniprot.org/core/author | "Scarafoni A."xsd:string |
http://purl.uniprot.org/citations/19962718 | http://purl.uniprot.org/core/author | "Scarafoni A."xsd:string |
http://purl.uniprot.org/citations/19962718 | http://purl.uniprot.org/core/author | "Ronchi A."xsd:string |
http://purl.uniprot.org/citations/19962718 | http://purl.uniprot.org/core/author | "Ronchi A."xsd:string |
http://purl.uniprot.org/citations/19962718 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
http://purl.uniprot.org/citations/19962718 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
http://purl.uniprot.org/citations/19962718 | http://purl.uniprot.org/core/name | "Phytochemistry"xsd:string |
http://purl.uniprot.org/citations/19962718 | http://purl.uniprot.org/core/name | "Phytochemistry"xsd:string |
http://purl.uniprot.org/citations/19962718 | http://purl.uniprot.org/core/pages | "142-148"xsd:string |
http://purl.uniprot.org/citations/19962718 | http://purl.uniprot.org/core/pages | "142-148"xsd:string |
http://purl.uniprot.org/citations/19962718 | http://purl.uniprot.org/core/title | "gamma-Conglutin, the Lupinus albus XEGIP-like protein, whose expression is elicited by chitosan, lacks of the typical inhibitory activity against GH12 endo-glucanases."xsd:string |
http://purl.uniprot.org/citations/19962718 | http://purl.uniprot.org/core/title | "gamma-Conglutin, the Lupinus albus XEGIP-like protein, whose expression is elicited by chitosan, lacks of the typical inhibitory activity against GH12 endo-glucanases."xsd:string |
http://purl.uniprot.org/citations/19962718 | http://purl.uniprot.org/core/volume | "71"xsd:string |
http://purl.uniprot.org/citations/19962718 | http://purl.uniprot.org/core/volume | "71"xsd:string |
http://purl.uniprot.org/citations/19962718 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19962718 |
http://purl.uniprot.org/citations/19962718 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19962718 |
http://purl.uniprot.org/citations/19962718 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19962718 |
http://purl.uniprot.org/citations/19962718 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19962718 |