| http://purl.uniprot.org/citations/19966073 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19966073 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19966073 | http://www.w3.org/2000/01/rdf-schema#comment | "The MRS2/MGT gene family in Arabidopsis thaliana belongs to the superfamily of CorA-MRS2-ALR-type membrane proteins. Proteins of this type are characterized by a GMN tripeptide motif (Gly-Met-Asn) at the end of the first of two C-terminal transmembrane domains and have been characterized as magnesium transporters. Using the recently established mag-fura-2 system allowing direct measurement of Mg(2+) uptake into mitochondria of Saccharomyces cerevisiae, we find that all members of the Arabidopsis family complement the corresponding yeast mrs2 mutant. Highly different patterns of tissue-specific expression were observed for the MRS2/MGT family members in planta. Six of them are expressed in root tissues, indicating a possible involvement in plant magnesium supply and distribution after uptake from the soil substrate. Homozygous T-DNA insertion knockout lines were obtained for four members of the MRS2/MGT gene family. A strong, magnesium-dependent phenotype of growth retardation was found for mrs2-7 when Mg(2+) concentrations were lowered to 50 microM in hydroponic cultures. Ectopic overexpression of MRS2-7 from the cauliflower mosaic virus 35S promoter results in complementation and increased biomass accumulation. Green fluorescent protein reporter gene fusions indicate a location of MRS2-7 in the endomembrane system. Hence, contrary to what is frequently found in analyses of plant gene families, a single gene family member knockout results in a strong, environmentally dependent phenotype."xsd:string |
| http://purl.uniprot.org/citations/19966073 | http://purl.org/dc/terms/identifier | "doi:10.1105/tpc.109.070557"xsd:string |
| http://purl.uniprot.org/citations/19966073 | http://purl.org/dc/terms/identifier | "doi:10.1105/tpc.109.070557"xsd:string |
| http://purl.uniprot.org/citations/19966073 | http://purl.uniprot.org/core/author | "Gebert M."xsd:string |
| http://purl.uniprot.org/citations/19966073 | http://purl.uniprot.org/core/author | "Gebert M."xsd:string |
| http://purl.uniprot.org/citations/19966073 | http://purl.uniprot.org/core/author | "Knoop V."xsd:string |
| http://purl.uniprot.org/citations/19966073 | http://purl.uniprot.org/core/author | "Knoop V."xsd:string |
| http://purl.uniprot.org/citations/19966073 | http://purl.uniprot.org/core/author | "Svidova S."xsd:string |
| http://purl.uniprot.org/citations/19966073 | http://purl.uniprot.org/core/author | "Svidova S."xsd:string |
| http://purl.uniprot.org/citations/19966073 | http://purl.uniprot.org/core/author | "Weghuber J."xsd:string |
| http://purl.uniprot.org/citations/19966073 | http://purl.uniprot.org/core/author | "Weghuber J."xsd:string |
| http://purl.uniprot.org/citations/19966073 | http://purl.uniprot.org/core/author | "Schweyen R."xsd:string |
| http://purl.uniprot.org/citations/19966073 | http://purl.uniprot.org/core/author | "Schweyen R."xsd:string |
| http://purl.uniprot.org/citations/19966073 | http://purl.uniprot.org/core/author | "Eifler K."xsd:string |
| http://purl.uniprot.org/citations/19966073 | http://purl.uniprot.org/core/author | "Eifler K."xsd:string |
| http://purl.uniprot.org/citations/19966073 | http://purl.uniprot.org/core/author | "Lenz H."xsd:string |
| http://purl.uniprot.org/citations/19966073 | http://purl.uniprot.org/core/author | "Lenz H."xsd:string |
| http://purl.uniprot.org/citations/19966073 | http://purl.uniprot.org/core/author | "Meschenmoser K."xsd:string |
| http://purl.uniprot.org/citations/19966073 | http://purl.uniprot.org/core/author | "Meschenmoser K."xsd:string |
| http://purl.uniprot.org/citations/19966073 | http://purl.uniprot.org/core/author | "Weyand K."xsd:string |
| http://purl.uniprot.org/citations/19966073 | http://purl.uniprot.org/core/author | "Weyand K."xsd:string |
| http://purl.uniprot.org/citations/19966073 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19966073 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |