| http://purl.uniprot.org/citations/19969597 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19969597 | http://www.w3.org/2000/01/rdf-schema#comment | "Recent studies highlighted an emerging possibility of using Drosophila as a model system for investigating the mechanisms of human congenital muscular dystrophies, called dystroglycanopathies, resulting from the abnormal glycosylation of alpha-dystroglycan. Several of these diseases are associated with defects in O-mannosylation, one of the most prominent types of alpha-dystroglycan glycosylation mediated by two protein O-mannosyltransferases. Drosophila appears to possess homologs of all essential components of the mammalian dystroglycan-mediated pathway; however, the glycosylation of Drosophila Dystroglycan (DG) has not yet been explored. In this study, we characterized the glycosylation of Drosophila DG using a combination of glycosidase treatments, lectin blots, trypsin digestion, and mass spectrometry analyses. Our results demonstrated that DG extracellular domain is O-mannosylated in vivo. We found that the concurrent in vivo activity of the two Drosophila protein O-mannosyltransferases, Rotated Abdomen and Twisted, is required for O-mannosylation of DG. While our experiments unambiguously determined some O-mannose sites far outside of the mucin-type domain of DG, they also provided evidence that DG bears a significant amount of O-mannosylation within its central region including the mucin-type domain, and that O-mannose can compete with O-GalNAc glycosylation of DG. We found that Rotated Abdomen and Twisted could potentiate in vivo the dominant-negative effect of DG extracellular domain expression on crossvein development, which suggests that O-mannosylation can modulate the ligand-binding activity of DG. Taken together these results demonstrated that O-mannosylation of Dystroglycan is an evolutionarily ancient mechanism conserved between Drosophila and humans, suggesting that Drosophila can be a suitable model system for studying molecular and genetic mechanisms underlying human dystroglycanopathies."xsd:string |
| http://purl.uniprot.org/citations/19969597 | http://purl.org/dc/terms/identifier | "doi:10.1093/glycob/cwp189"xsd:string |
| http://purl.uniprot.org/citations/19969597 | http://purl.uniprot.org/core/author | "Nakamura N."xsd:string |
| http://purl.uniprot.org/citations/19969597 | http://purl.uniprot.org/core/author | "Wells L."xsd:string |
| http://purl.uniprot.org/citations/19969597 | http://purl.uniprot.org/core/author | "Stalnaker S.H."xsd:string |
| http://purl.uniprot.org/citations/19969597 | http://purl.uniprot.org/core/author | "Panin V.M."xsd:string |
| http://purl.uniprot.org/citations/19969597 | http://purl.uniprot.org/core/author | "Lyalin D."xsd:string |
| http://purl.uniprot.org/citations/19969597 | http://purl.uniprot.org/core/author | "Lavrova O."xsd:string |
| http://purl.uniprot.org/citations/19969597 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/19969597 | http://purl.uniprot.org/core/name | "Glycobiology"xsd:string |
| http://purl.uniprot.org/citations/19969597 | http://purl.uniprot.org/core/pages | "381-394"xsd:string |
| http://purl.uniprot.org/citations/19969597 | http://purl.uniprot.org/core/title | "Drosophila Dystroglycan is a target of O-mannosyltransferase activity of two protein O-mannosyltransferases, Rotated Abdomen and Twisted."xsd:string |
| http://purl.uniprot.org/citations/19969597 | http://purl.uniprot.org/core/volume | "20"xsd:string |
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