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http://purl.uniprot.org/citations/19995930http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19995930http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19995930http://www.w3.org/2000/01/rdf-schema#comment"TEM-24 remains one of the most widespread TEM-type extended-spectrum beta-lactamases (ESBLs) among Enterobacteriaceae. To analyze the reasons influencing its spread and persistence, a multilevel population genetics study was carried out on 28 representative TEM-24 producers from Belgium, France, Portugal, and Spain (13 Enterobacter aerogenes isolates, 6 Escherichia coli isolates, 6 Klebsiella pneumoniae isolates, 2 Proteus mirabilis isolates, and 1 Klebsiella oxytoca isolate, from 1998 to 2004). Clonal relatedness (XbaI pulsed-field gel electrophoresis [PFGE] and E. coli phylogroups) and antibiotic susceptibility were determined by standard procedures. Plasmid analysis included determination of the incompatibility group (by PCR, hybridization, and/or sequencing) and comparison of restriction fragment length polymorphism (RFLP) patterns. Characterization of genetic elements conferring antibiotic resistance included integrons (classes 1, 2, and 3) and transposons (Tn3, Tn21, and Tn402). Similar PFGE patterns were identified among E. aerogenes, K. pneumoniae, and P. mirabilis isolates, while E. coli strains were diverse (phylogenetic groups A, B2, and D). Highly related 180-kb IncA/C2 plasmids conferring resistance to kanamycin, tobramycin, chloramphenicol, trimethoprim, and sulfonamides were identified. Each plasmid contained defective In0-Tn402 (dfrA1-aadA1, aacA4, or aacA4-aacC1-orfE-aadA2-cmlA1) and In4-Tn402 (aacA4 or dfrA1-aadA1) variants. These integrons were located within Tn21, Tn1696, or hybrids of these transposons, with IS5075 interrupting their IRtnp and IRmer. In all cases, blaTEM-24 was part of an IS5075-DeltaTn1 transposon within tnp1696, mimicking other genetic elements containing blaTEM-2 and blaTEM-3 variants. The international dissemination of TEM-24 is fuelled by an IncA/C2 plasmid acquired by different enterobacterial clones which seem to evolve by gaining diverse genetic elements. This work highlights the risks of a confluence between highly penetrating clones and highly promiscuous plasmids in the spread of antibiotic resistance, and it contributes to the elucidation of the origin and evolution of TEM-2 ESBL derivatives."xsd:string
http://purl.uniprot.org/citations/19995930http://purl.org/dc/terms/identifier"doi:10.1128/AAC.00959-09"xsd:string
http://purl.uniprot.org/citations/19995930http://purl.org/dc/terms/identifier"doi:10.1128/aac.00959-09"xsd:string
http://purl.uniprot.org/citations/19995930http://purl.uniprot.org/core/author"Baquero F."xsd:string
http://purl.uniprot.org/citations/19995930http://purl.uniprot.org/core/author"Baquero F."xsd:string
http://purl.uniprot.org/citations/19995930http://purl.uniprot.org/core/author"Machado E."xsd:string
http://purl.uniprot.org/citations/19995930http://purl.uniprot.org/core/author"Machado E."xsd:string
http://purl.uniprot.org/citations/19995930http://purl.uniprot.org/core/author"Peixe L."xsd:string
http://purl.uniprot.org/citations/19995930http://purl.uniprot.org/core/author"Peixe L."xsd:string
http://purl.uniprot.org/citations/19995930http://purl.uniprot.org/core/author"Canton R."xsd:string
http://purl.uniprot.org/citations/19995930http://purl.uniprot.org/core/author"Canton R."xsd:string
http://purl.uniprot.org/citations/19995930http://purl.uniprot.org/core/author"Novais A."xsd:string
http://purl.uniprot.org/citations/19995930http://purl.uniprot.org/core/author"Novais A."xsd:string
http://purl.uniprot.org/citations/19995930http://purl.uniprot.org/core/author"Coque T.M."xsd:string
http://purl.uniprot.org/citations/19995930http://purl.uniprot.org/core/author"Coque T.M."xsd:string
http://purl.uniprot.org/citations/19995930http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/19995930http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/19995930http://purl.uniprot.org/core/name"Antimicrob. Agents Chemother."xsd:string
http://purl.uniprot.org/citations/19995930http://purl.uniprot.org/core/name"Antimicrob Agents Chemother"xsd:string
http://purl.uniprot.org/citations/19995930http://purl.uniprot.org/core/pages"825-834"xsd:string
http://purl.uniprot.org/citations/19995930http://purl.uniprot.org/core/pages"825-834"xsd:string
http://purl.uniprot.org/citations/19995930http://purl.uniprot.org/core/title"International spread and persistence of TEM-24 is caused by the confluence of highly penetrating enterobacteriaceae clones and an IncA/C2 plasmid containing Tn1696::Tn1 and IS5075-Tn21."xsd:string
http://purl.uniprot.org/citations/19995930http://purl.uniprot.org/core/title"International spread and persistence of TEM-24 is caused by the confluence of highly penetrating enterobacteriaceae clones and an IncA/C2 plasmid containing Tn1696::Tn1 and IS5075-Tn21."xsd:string
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