| http://purl.uniprot.org/citations/20023122 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20023122 | http://www.w3.org/2000/01/rdf-schema#comment | "Rewarming patients after profound hypothermia may result in acute heart failure and high mortality (50-80%). However, the underlying pathophysiological mechanisms are largely unknown. We characterized cardiac contractile function in the temperature range of 15-30 degrees C by measuring the intracellular Ca(2+) concentration ([Ca(2+)](i)) and twitch force in intact left ventricular rat papillary muscles. Muscle preparations were loaded with fura-2 AM and electrically stimulated during cooling at 15 degrees C for 1.5 h before being rewarmed to the baseline temperature of 30 degrees C. After hypothermia/rewarming, peak twitch force decreased by 30-40%, but [Ca(2+)](i) was not significantly altered. In addition, we assessed the maximal Ca(2+)-activated force (F(max)) and Ca(2+) sensitivity of force in skinned papillary muscle fibers. F(max) was decreased by approximately 30%, whereas the pCa required for 50% of F(max) was reduced by approximately 0.14. In rewarmed papillary muscle, both total cardiac troponin I (cTnI) phosphorylation and PKA-mediated cTnI phosphorylation at Ser23/24 were significantly increased compared with controls. We conclude that after hypothermia/rewarming, myocardial contractility is significantly reduced, as evidenced by reduced twitch force and F(max). The reduced myocardial contractility is attributed to decreased Ca(2+) sensitivity of force rather than [Ca(2+)](i) itself, resulting from increased cTnI phosphorylation."xsd:string |
| http://purl.uniprot.org/citations/20023122 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajpheart.00805.2009"xsd:string |
| http://purl.uniprot.org/citations/20023122 | http://purl.uniprot.org/core/author | "Han Y.S."xsd:string |
| http://purl.uniprot.org/citations/20023122 | http://purl.uniprot.org/core/author | "Prakash Y.S."xsd:string |
| http://purl.uniprot.org/citations/20023122 | http://purl.uniprot.org/core/author | "Sieck G.C."xsd:string |
| http://purl.uniprot.org/citations/20023122 | http://purl.uniprot.org/core/author | "Tveita T."xsd:string |
| http://purl.uniprot.org/citations/20023122 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20023122 | http://purl.uniprot.org/core/name | "Am J Physiol Heart Circ Physiol"xsd:string |
| http://purl.uniprot.org/citations/20023122 | http://purl.uniprot.org/core/pages | "H890-7"xsd:string |
| http://purl.uniprot.org/citations/20023122 | http://purl.uniprot.org/core/title | "Mechanisms underlying hypothermia-induced cardiac contractile dysfunction."xsd:string |
| http://purl.uniprot.org/citations/20023122 | http://purl.uniprot.org/core/volume | "298"xsd:string |
| http://purl.uniprot.org/citations/20023122 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20023122 |
| http://purl.uniprot.org/citations/20023122 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20023122 |
| http://purl.uniprot.org/uniprot/#_P19429-mappedCitation-20023122 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20023122 |
| http://purl.uniprot.org/uniprot/P19429 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20023122 |