| http://purl.uniprot.org/citations/20026641 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20026641 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20026641 | http://www.w3.org/2000/01/rdf-schema#comment | "Disruption of the gene encoding protein tyrosine kinase 6 (PTK6) leads to increased growth, impaired enterocyte differentiation and higher levels of nuclear beta-catenin in the mouse small intestine. Here, we demonstrate that PTK6 associates with nuclear and cytoplasmic beta-catenin and inhibits beta-catenin- and T-cell factor (TCF)-mediated transcription. PTK6 directly phosphorylates beta-catenin on Tyr64, Tyr142, Tyr331 and/or Tyr333, with the predominant site being Tyr64. However, mutation of these sites does not abrogate the ability of PTK6 to inhibit beta-catenin transcriptional activity. Outcomes of PTK6-mediated regulation appear to be dependent on its intracellular localization. In the SW620 colorectal adenocarcinoma cell line, nuclear-targeted PTK6 negatively regulates endogenous beta-catenin/TCF transcriptional activity, whereas membrane-targeted PTK6 enhances beta-catenin/TCF regulated transcription. Levels of TCF4 and the transcriptional co-repressor TLE/Groucho increase in SW620 cells expressing nuclear-targeted PTK6. Knockdown of PTK6 in SW620 cells leads to increased beta-catenin/TCF transcriptional activity and increased expression of beta-catenin/TCF target genes Myc and Survivin. Ptk6-null BAT-GAL mice, containing a beta-catenin-activated LacZ reporter transgene, have increased levels of beta-galactosidase expression in the gastrointestinal tract. The ability of PTK6 to negatively regulate beta-catenin/TCF transcription by modulating levels of TCF4 and TLE/Groucho could contribute to its growth-inhibitory activities in vivo."xsd:string |
| http://purl.uniprot.org/citations/20026641 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.053264"xsd:string |
| http://purl.uniprot.org/citations/20026641 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.053264"xsd:string |
| http://purl.uniprot.org/citations/20026641 | http://purl.uniprot.org/core/author | "Asara J.M."xsd:string |
| http://purl.uniprot.org/citations/20026641 | http://purl.uniprot.org/core/author | "Asara J.M."xsd:string |
| http://purl.uniprot.org/citations/20026641 | http://purl.uniprot.org/core/author | "Zheng Y."xsd:string |
| http://purl.uniprot.org/citations/20026641 | http://purl.uniprot.org/core/author | "Zheng Y."xsd:string |
| http://purl.uniprot.org/citations/20026641 | http://purl.uniprot.org/core/author | "Bie W."xsd:string |
| http://purl.uniprot.org/citations/20026641 | http://purl.uniprot.org/core/author | "Bie W."xsd:string |
| http://purl.uniprot.org/citations/20026641 | http://purl.uniprot.org/core/author | "Brauer P.M."xsd:string |
| http://purl.uniprot.org/citations/20026641 | http://purl.uniprot.org/core/author | "Brauer P.M."xsd:string |
| http://purl.uniprot.org/citations/20026641 | http://purl.uniprot.org/core/author | "Gierut J.J."xsd:string |
| http://purl.uniprot.org/citations/20026641 | http://purl.uniprot.org/core/author | "Gierut J.J."xsd:string |
| http://purl.uniprot.org/citations/20026641 | http://purl.uniprot.org/core/author | "Palka-Hamblin H.L."xsd:string |
| http://purl.uniprot.org/citations/20026641 | http://purl.uniprot.org/core/author | "Palka-Hamblin H.L."xsd:string |
| http://purl.uniprot.org/citations/20026641 | http://purl.uniprot.org/core/author | "Tyner A.L."xsd:string |
| http://purl.uniprot.org/citations/20026641 | http://purl.uniprot.org/core/author | "Tyner A.L."xsd:string |
| http://purl.uniprot.org/citations/20026641 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20026641 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20026641 | http://purl.uniprot.org/core/name | "J. Cell Sci."xsd:string |
| http://purl.uniprot.org/citations/20026641 | http://purl.uniprot.org/core/name | "J. Cell Sci."xsd:string |
| http://purl.uniprot.org/citations/20026641 | http://purl.uniprot.org/core/pages | "236-245"xsd:string |
| http://purl.uniprot.org/citations/20026641 | http://purl.uniprot.org/core/pages | "236-245"xsd:string |