| http://purl.uniprot.org/citations/2002850 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2002850 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2002850 | http://www.w3.org/2000/01/rdf-schema#comment | "Galactose oxidase is an extracellular enzyme secreted by the fungus Dactylium dendroides. It is monomeric, with a relative molecular mass of 68,000, catalyses the stereospecific oxidation of a broad range of primary alcohol substrates and possesses a unique mononuclear copper site essential for catalysing a two-electron transfer reaction during the oxidation of primary alcohols to corresponding aldehydes. Recent evidence arguing against a Cu(III)-Cu(I) couple implies the existence of a second redox-active site proposed to involve pyrroloquinoline quinone or a tyrosine radical. We now report the crystal structure of galactose oxidase at 1.7 A resolution. This reveals a unique structural feature at the copper site with a novel thioether bond linking Cys 228 and Tyr 272 in a stacking interaction with Trp 290. We propose that these molecular components stabilize the protein free-radical species essential for catalysis and thus provide a 'built-in' secondary cofactor. This feature may represent a new mechanism for mediating electron transfer in metalloenzymes in the absence of exogenous cofactors."xsd:string |
| http://purl.uniprot.org/citations/2002850 | http://purl.org/dc/terms/identifier | "doi:10.1038/350087a0"xsd:string |
| http://purl.uniprot.org/citations/2002850 | http://purl.org/dc/terms/identifier | "doi:10.1038/350087a0"xsd:string |
| http://purl.uniprot.org/citations/2002850 | http://purl.uniprot.org/core/author | "Keen J.N."xsd:string |
| http://purl.uniprot.org/citations/2002850 | http://purl.uniprot.org/core/author | "Keen J.N."xsd:string |
| http://purl.uniprot.org/citations/2002850 | http://purl.uniprot.org/core/author | "Ito N."xsd:string |
| http://purl.uniprot.org/citations/2002850 | http://purl.uniprot.org/core/author | "Ito N."xsd:string |
| http://purl.uniprot.org/citations/2002850 | http://purl.uniprot.org/core/author | "Phillips S.E.V."xsd:string |
| http://purl.uniprot.org/citations/2002850 | http://purl.uniprot.org/core/author | "Phillips S.E.V."xsd:string |
| http://purl.uniprot.org/citations/2002850 | http://purl.uniprot.org/core/author | "Knowles P.F."xsd:string |
| http://purl.uniprot.org/citations/2002850 | http://purl.uniprot.org/core/author | "Knowles P.F."xsd:string |
| http://purl.uniprot.org/citations/2002850 | http://purl.uniprot.org/core/author | "McPherson M.J."xsd:string |
| http://purl.uniprot.org/citations/2002850 | http://purl.uniprot.org/core/author | "McPherson M.J."xsd:string |
| http://purl.uniprot.org/citations/2002850 | http://purl.uniprot.org/core/author | "Yadav K.D.S."xsd:string |
| http://purl.uniprot.org/citations/2002850 | http://purl.uniprot.org/core/author | "Yadav K.D.S."xsd:string |
| http://purl.uniprot.org/citations/2002850 | http://purl.uniprot.org/core/author | "Ogel Z.B."xsd:string |
| http://purl.uniprot.org/citations/2002850 | http://purl.uniprot.org/core/author | "Ogel Z.B."xsd:string |
| http://purl.uniprot.org/citations/2002850 | http://purl.uniprot.org/core/author | "Stevens C.E."xsd:string |
| http://purl.uniprot.org/citations/2002850 | http://purl.uniprot.org/core/author | "Stevens C.E."xsd:string |
| http://purl.uniprot.org/citations/2002850 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/2002850 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/2002850 | http://purl.uniprot.org/core/name | "Nature"xsd:string |
| http://purl.uniprot.org/citations/2002850 | http://purl.uniprot.org/core/name | "Nature"xsd:string |