| http://purl.uniprot.org/citations/20042668 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20042668 | http://www.w3.org/2000/01/rdf-schema#comment | "Remodeling of the stromal extracellular matrix and elevated expression of specific proto-oncogenes within the adjacent epithelium represent cardinal features of breast cancer, yet how these events become integrated is not fully understood. To address this question, we focused on tenascin-C (TN-C), a stromal extracellular matrix glycoprotein whose expression increases with disease severity. Initially, nonmalignant human mammary epithelial cells (MCF-10A) were cultured within a reconstituted basement membrane (BM) where they formed three-dimensional (3-D) polarized, growth-attenuated, multicellular acini, enveloped by a continuous endogenous BM. In the presence of TN-C, however, acini failed to generate a normal BM, and net epithelial cell proliferation increased. To quantify how TN-C alters 3-D tissue architecture and function, we developed a computational image analysis algorithm, which showed that although TN-C disrupted acinar surface structure, it had no effect on their volume. Thus, TN-C promoted epithelial cell proliferation leading to luminal filling, a process that we hypothesized involved c-met, a proto-oncogene amplified in breast tumors that promotes intraluminal filling. Indeed, TN-C increased epithelial c-met expression and promoted luminal filling, whereas blockade of c-met function reversed this phenotype, resulting in normal BM deposition, proper lumen formation, and decreased cell proliferation. Collectively, these studies, combining a novel quantitative image analysis tool with 3-D organotypic cultures, demonstrate that stromal changes associated with breast cancer can control proto-oncogene function."xsd:string |
| http://purl.uniprot.org/citations/20042668 | http://purl.org/dc/terms/identifier | "doi:10.2353/ajpath.2010.090006"xsd:string |
| http://purl.uniprot.org/citations/20042668 | http://purl.uniprot.org/core/author | "Jones P.L."xsd:string |
| http://purl.uniprot.org/citations/20042668 | http://purl.uniprot.org/core/author | "Schedin P."xsd:string |
| http://purl.uniprot.org/citations/20042668 | http://purl.uniprot.org/core/author | "Taraseviciute A."xsd:string |
| http://purl.uniprot.org/citations/20042668 | http://purl.uniprot.org/core/author | "Vincent B.T."xsd:string |
| http://purl.uniprot.org/citations/20042668 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20042668 | http://purl.uniprot.org/core/name | "Am J Pathol"xsd:string |
| http://purl.uniprot.org/citations/20042668 | http://purl.uniprot.org/core/pages | "827-838"xsd:string |
| http://purl.uniprot.org/citations/20042668 | http://purl.uniprot.org/core/title | "Quantitative analysis of three-dimensional human mammary epithelial tissue architecture reveals a role for tenascin-C in regulating c-met function."xsd:string |
| http://purl.uniprot.org/citations/20042668 | http://purl.uniprot.org/core/volume | "176"xsd:string |
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