| http://purl.uniprot.org/citations/20048156 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20048156 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20048156 | http://www.w3.org/2000/01/rdf-schema#comment | "CmCatB, a cathepsin B-type cysteine protease, is insensitive to inhibition by the soybean cysteine protease inhibitor (scN). Cowpea bruchids dramatically induce CmCatB expression when major digestive proteases are inactivated by dietary scN, which is presumably an adaptive strategy that insects use to minimize effects of nutrient deficiency. In this study, we cloned the cowpea bruchid hepatocyte nuclear factor 4 (CmHNF-4) and demonstrated its involvement in transcriptional activation of CmCatB in the digestive tract of scN-adapted bruchids. Electrophoretic mobility shift assays demonstrated that CmHNF-4 binds to a CmCatB promoter region containing two tandem chicken ovalbumin upstream promoter (COUP) sites, which is also the cis-element for Seven-up (CmSvp), a previously identified transcriptional repressor of CmCatB. Although CmSvp is predominantly expressed in unadapted insect midgut, CmHNF-4 is more abundant in adapted bruchids. When transiently expressed in Drosophila S2 cells, CmHNF-4 substantially increased CmCatB expression through COUP binding. CmSvp inhibited CmHNF-4-mediated transcriptional activation even in the absence of its DNA-binding domain. Thus antagonism resulted, at least in part, from protein-protein interactions between CmSvp and CmHNF-4. Association of the two transcription factors was subsequently confirmed by glutathione S-transferase pulldown assays. Interestingly, anti-CmHNF-4 serum caused a supershift not only with nuclear extracts of scN-adapted insect midgut but with that of unadapted control insects as well. The presence of CmHNF-4 in unadapted insects further supported the idea that interplay between CmSvp and CmHNF-4 controls CmCatB transcription activation. Together, these results suggest that coordination between CmHNF-4 and CmSvp is important in counter-defense gene regulation in insects."xsd:string |
| http://purl.uniprot.org/citations/20048156 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.M109.095596"xsd:string |
| http://purl.uniprot.org/citations/20048156 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m109.095596"xsd:string |
| http://purl.uniprot.org/citations/20048156 | http://purl.uniprot.org/core/author | "Guarino L.A."xsd:string |
| http://purl.uniprot.org/citations/20048156 | http://purl.uniprot.org/core/author | "Guarino L.A."xsd:string |
| http://purl.uniprot.org/citations/20048156 | http://purl.uniprot.org/core/author | "Ahn J.E."xsd:string |
| http://purl.uniprot.org/citations/20048156 | http://purl.uniprot.org/core/author | "Ahn J.E."xsd:string |
| http://purl.uniprot.org/citations/20048156 | http://purl.uniprot.org/core/author | "Zhu-Salzman K."xsd:string |
| http://purl.uniprot.org/citations/20048156 | http://purl.uniprot.org/core/author | "Zhu-Salzman K."xsd:string |
| http://purl.uniprot.org/citations/20048156 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20048156 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20048156 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/20048156 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/20048156 | http://purl.uniprot.org/core/pages | "6573-6584"xsd:string |
| http://purl.uniprot.org/citations/20048156 | http://purl.uniprot.org/core/pages | "6573-6584"xsd:string |
| http://purl.uniprot.org/citations/20048156 | http://purl.uniprot.org/core/title | "Coordination of hepatocyte nuclear factor 4 and seven-up controls insect counter-defense cathepsin B expression."xsd:string |
| http://purl.uniprot.org/citations/20048156 | http://purl.uniprot.org/core/title | "Coordination of hepatocyte nuclear factor 4 and seven-up controls insect counter-defense cathepsin B expression."xsd:string |
| http://purl.uniprot.org/citations/20048156 | http://purl.uniprot.org/core/volume | "285"xsd:string |
| http://purl.uniprot.org/citations/20048156 | http://purl.uniprot.org/core/volume | "285"xsd:string |
| http://purl.uniprot.org/citations/20048156 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20048156 |
| http://purl.uniprot.org/citations/20048156 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20048156 |
| http://purl.uniprot.org/citations/20048156 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20048156 |
| http://purl.uniprot.org/citations/20048156 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20048156 |