| http://purl.uniprot.org/citations/20048345 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20048345 | http://www.w3.org/2000/01/rdf-schema#comment | "Surfactant protein A (SP-A) plays a role in lung innate immunity and surfactant-related functions. Two functional genes, SP-A1 (SFTPA1) and SP-A2 (SFTPA2), are present in humans and primates (rodents have one gene). Single gene SP-A1 or SP-A2 proteins expressed in vitro are functional. To study their role in vivo, we generated humanized transgenic (hTG) C57BL/6 mice, SP-A1(6A(4)) and SP-A2(1A(3)). The SP-A cDNA in experimental constructs was driven by the 3.7-kb SP-C promoter. Positive hTG mice were bred with SP-A knock-out mice to generate F8 offspring for study. Epithelial alveolar type II cells were SP-A-positive, and Clara cells were negative by immunohistochemistry in hTG mice. The levels of SP-A in lungs of two hTG lines used were comparable with those in human lungs. Southern blot analysis indicated that two cDNA copies of either SP-A1(6A(4)) or SP-A2(1A(3)) were integrated as a concatemer into the genome of each of the two hTG lines. Electron microscopy analysis revealed that hTG mice with a single SP-A1(6A(4)) or SP-A2(1A(3)) gene product lacked tubular myelin (TM), but hTG mice carrying both had TM. Furthermore, TM was observed in human bronchoalveolar lavage fluid only if both SP-A1 and SP-A2 gene products were present and not in those containing primarily (>99.7%) either SP-A1 or SP-A2 gene products. In vivo rescue study confirmed that TM can only be restored after administering exogenous SP-A containing both SP-A1 and SP-A2 into the lungs of SP-A knock-out mice. These observations indicate that SP-A1 and SP-A2 diverged functionally at least in terms of TM formation."xsd:string |
| http://purl.uniprot.org/citations/20048345 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m109.046243"xsd:string |
| http://purl.uniprot.org/citations/20048345 | http://purl.uniprot.org/core/author | "Guo X."xsd:string |
| http://purl.uniprot.org/citations/20048345 | http://purl.uniprot.org/core/author | "Wang G."xsd:string |
| http://purl.uniprot.org/citations/20048345 | http://purl.uniprot.org/core/author | "Floros J."xsd:string |
| http://purl.uniprot.org/citations/20048345 | http://purl.uniprot.org/core/author | "Thomas N.J."xsd:string |
| http://purl.uniprot.org/citations/20048345 | http://purl.uniprot.org/core/author | "Diangelo S."xsd:string |
| http://purl.uniprot.org/citations/20048345 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20048345 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/20048345 | http://purl.uniprot.org/core/pages | "11998-12010"xsd:string |
| http://purl.uniprot.org/citations/20048345 | http://purl.uniprot.org/core/title | "Humanized SFTPA1 and SFTPA2 transgenic mice reveal functional divergence of SP-A1 and SP-A2: formation of tubular myelin in vivo requires both gene products."xsd:string |
| http://purl.uniprot.org/citations/20048345 | http://purl.uniprot.org/core/volume | "285"xsd:string |
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