| http://purl.uniprot.org/citations/20064504 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20064504 | http://www.w3.org/2000/01/rdf-schema#comment | "Loss of Omi/HtrA2 function leads to nerve cell loss in mouse models and has been linked to neurodegeneration in Parkinson's and Huntington's disease. Omi/HtrA2 is a serine protease released as a pro-apoptotic factor from the mitochondrial intermembrane space into the cytosol. Under physiological conditions, Omi/HtrA2 is thought to be involved in protection against cellular stress, but the cytological and molecular mechanisms are not clear. Omi/HtrA2 deficiency caused an accumulation of reactive oxygen species and reduced mitochondrial membrane potential. In Omi/HtrA2 knockout mouse embryonic fibroblasts, as well as in Omi/HtrA2 silenced human HeLa cells and Drosophila S2R+ cells, we found elongated mitochondria by live cell imaging. Electron microscopy confirmed the mitochondrial morphology alterations and showed abnormal cristae structure. Examining the levels of proteins involved in mitochondrial fusion, we found a selective up-regulation of more soluble OPA1 protein. Complementation of knockout cells with wild-type Omi/HtrA2 but not with the protease mutant [S306A]Omi/HtrA2 reversed the mitochondrial elongation phenotype and OPA1 alterations. Finally, co-immunoprecipitation showed direct interaction of Omi/HtrA2 with endogenous OPA1. Thus, we show for the first time a direct effect of loss of Omi/HtrA2 on mitochondrial morphology and demonstrate a novel role of this mitochondrial serine protease in the modulation of OPA1. Our results underscore a critical role of impaired mitochondrial dynamics in neurodegenerative disorders."xsd:string |
| http://purl.uniprot.org/citations/20064504 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.yexcr.2010.01.005"xsd:string |
| http://purl.uniprot.org/citations/20064504 | http://purl.uniprot.org/core/author | "Whitworth A.J."xsd:string |
| http://purl.uniprot.org/citations/20064504 | http://purl.uniprot.org/core/author | "Wolburg H."xsd:string |
| http://purl.uniprot.org/citations/20064504 | http://purl.uniprot.org/core/author | "Kruger R."xsd:string |
| http://purl.uniprot.org/citations/20064504 | http://purl.uniprot.org/core/author | "Fiesel F.C."xsd:string |
| http://purl.uniprot.org/citations/20064504 | http://purl.uniprot.org/core/author | "Ciceri D."xsd:string |
| http://purl.uniprot.org/citations/20064504 | http://purl.uniprot.org/core/author | "Ziviani E."xsd:string |
| http://purl.uniprot.org/citations/20064504 | http://purl.uniprot.org/core/author | "Martins L.M."xsd:string |
| http://purl.uniprot.org/citations/20064504 | http://purl.uniprot.org/core/author | "Kahle P.J."xsd:string |
| http://purl.uniprot.org/citations/20064504 | http://purl.uniprot.org/core/author | "Kieper N."xsd:string |
| http://purl.uniprot.org/citations/20064504 | http://purl.uniprot.org/core/author | "Holmstrom K.M."xsd:string |
| http://purl.uniprot.org/citations/20064504 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20064504 | http://purl.uniprot.org/core/name | "Exp Cell Res"xsd:string |
| http://purl.uniprot.org/citations/20064504 | http://purl.uniprot.org/core/pages | "1213-1224"xsd:string |
| http://purl.uniprot.org/citations/20064504 | http://purl.uniprot.org/core/title | "Modulation of mitochondrial function and morphology by interaction of Omi/HtrA2 with the mitochondrial fusion factor OPA1."xsd:string |
| http://purl.uniprot.org/citations/20064504 | http://purl.uniprot.org/core/volume | "316"xsd:string |
| http://purl.uniprot.org/citations/20064504 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20064504 |
| http://purl.uniprot.org/citations/20064504 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20064504 |
| http://purl.uniprot.org/uniprot/#_D3YX27-mappedCitation-20064504 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20064504 |
| http://purl.uniprot.org/uniprot/#_D3YX28-mappedCitation-20064504 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20064504 |
| http://purl.uniprot.org/uniprot/#_E0CXD1-mappedCitation-20064504 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20064504 |
| http://purl.uniprot.org/uniprot/#_F6U775-mappedCitation-20064504 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20064504 |
| http://purl.uniprot.org/uniprot/#_F6XUR8-mappedCitation-20064504 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20064504 |