| http://purl.uniprot.org/citations/20068230 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20068230 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20068230 | http://www.w3.org/2000/01/rdf-schema#comment | "Like phosphorylation, the addition of O-linked beta-N-acetylglucosamine (O-GlcNAcylation) is a ubiquitous, reversible process that modifies serine and threonine residues on nuclear and cytoplasmic proteins. Overexpression of the enzyme that adds O-GlcNAc to target proteins, O-GlcNAc transferase (OGT), perturbs cytokinesis and promotes polyploidy, but the molecular targets of OGT that are important for its cell cycle functions are unknown. Here, we identify 141 previously unknown O-GlcNAc sites on proteins that function in spindle assembly and cytokinesis. Many of these O-GlcNAcylation sites are either identical to known phosphorylation sites or in close proximity to them. Furthermore, we found that O-GlcNAcylation altered the phosphorylation of key proteins associated with the mitotic spindle and midbody. Forced overexpression of OGT increased the inhibitory phosphorylation of cyclin-dependent kinase 1 (CDK1) and reduced the phosphorylation of CDK1 target proteins. The increased phosphorylation of CDK1 is explained by increased activation of its upstream kinase, MYT1, and by a concomitant reduction in the transcript for the CDK1 phosphatase, CDC25C. OGT overexpression also caused a reduction in both messenger RNA expression and protein abundance of Polo-like kinase 1, which is upstream of both MYT1 and CDC25C. The data not only illustrate the crosstalk between O-GlcNAcylation and phosphorylation of proteins that are regulators of crucial signaling pathways but also uncover a mechanism for the role of O-GlcNAcylation in regulation of cell division."xsd:string |
| http://purl.uniprot.org/citations/20068230 | http://purl.org/dc/terms/identifier | "doi:10.1126/scisignal.2000526"xsd:string |
| http://purl.uniprot.org/citations/20068230 | http://purl.org/dc/terms/identifier | "doi:10.1126/scisignal.2000526"xsd:string |
| http://purl.uniprot.org/citations/20068230 | http://purl.uniprot.org/core/author | "Hunt D.F."xsd:string |
| http://purl.uniprot.org/citations/20068230 | http://purl.uniprot.org/core/author | "Hunt D.F."xsd:string |
| http://purl.uniprot.org/citations/20068230 | http://purl.uniprot.org/core/author | "Shabanowitz J."xsd:string |
| http://purl.uniprot.org/citations/20068230 | http://purl.uniprot.org/core/author | "Shabanowitz J."xsd:string |
| http://purl.uniprot.org/citations/20068230 | http://purl.uniprot.org/core/author | "Wang Z."xsd:string |
| http://purl.uniprot.org/citations/20068230 | http://purl.uniprot.org/core/author | "Wang Z."xsd:string |
| http://purl.uniprot.org/citations/20068230 | http://purl.uniprot.org/core/author | "Hart G.W."xsd:string |
| http://purl.uniprot.org/citations/20068230 | http://purl.uniprot.org/core/author | "Hart G.W."xsd:string |
| http://purl.uniprot.org/citations/20068230 | http://purl.uniprot.org/core/author | "Cheung W.D."xsd:string |
| http://purl.uniprot.org/citations/20068230 | http://purl.uniprot.org/core/author | "Cheung W.D."xsd:string |
| http://purl.uniprot.org/citations/20068230 | http://purl.uniprot.org/core/author | "Sakabe K."xsd:string |
| http://purl.uniprot.org/citations/20068230 | http://purl.uniprot.org/core/author | "Sakabe K."xsd:string |
| http://purl.uniprot.org/citations/20068230 | http://purl.uniprot.org/core/author | "Udeshi N.D."xsd:string |
| http://purl.uniprot.org/citations/20068230 | http://purl.uniprot.org/core/author | "Udeshi N.D."xsd:string |
| http://purl.uniprot.org/citations/20068230 | http://purl.uniprot.org/core/author | "Slawson C."xsd:string |
| http://purl.uniprot.org/citations/20068230 | http://purl.uniprot.org/core/author | "Slawson C."xsd:string |
| http://purl.uniprot.org/citations/20068230 | http://purl.uniprot.org/core/author | "Compton P.D."xsd:string |
| http://purl.uniprot.org/citations/20068230 | http://purl.uniprot.org/core/author | "Compton P.D."xsd:string |
| http://purl.uniprot.org/citations/20068230 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20068230 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |