| http://purl.uniprot.org/citations/20080593 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20080593 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20080593 | http://www.w3.org/2000/01/rdf-schema#comment | "RNA virus infection is recognized by retinoic acid-inducible gene (RIG)-I-like receptors (RLRs), RIG-I, and melanoma differentiation-associated gene 5 (MDA5) in the cytoplasm. RLRs are comprised of N-terminal caspase-recruitment domains (CARDs) and a DExD/H-box helicase domain. The third member of the RLR family, LGP2, lacks any CARDs and was originally identified as a negative regulator of RLR signaling. In the present study, we generated mice lacking LGP2 and found that LGP2 was required for RIG-I- and MDA5-mediated antiviral responses. In particular, LGP2 was essential for type I IFN production in response to picornaviridae infection. Overexpression of the CARDs from RIG-I and MDA5 in Lgp2(-/-) fibroblasts activated the IFN-beta promoter, suggesting that LGP2 acts upstream of RIG-I and MDA5. We further examined the role of the LGP2 helicase domain by generating mice harboring a point mutation of Lys-30 to Ala (Lgp2 (K30A/K30A)) that abrogated the LGP2 ATPase activity. Lgp2 (K30A/K30A) dendritic cells showed impaired IFN-beta productions in response to various RNA viruses to extents similar to those of Lgp2(-/-) cells. Lgp2(-/-) and Lgp2 (K30A/K30A) mice were highly susceptible to encephalomyocarditis virus infection. Nevertheless, LGP2 and its ATPase activity were dispensable for the responses to synthetic RNA ligands for MDA5 and RIG-I. Taken together, the present data suggest that LGP2 facilitates viral RNA recognition by RIG-I and MDA5 through its ATPase domain."xsd:string |
| http://purl.uniprot.org/citations/20080593 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.0912986107"xsd:string |
| http://purl.uniprot.org/citations/20080593 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.0912986107"xsd:string |
| http://purl.uniprot.org/citations/20080593 | http://purl.uniprot.org/core/author | "Kato H."xsd:string |
| http://purl.uniprot.org/citations/20080593 | http://purl.uniprot.org/core/author | "Kato H."xsd:string |
| http://purl.uniprot.org/citations/20080593 | http://purl.uniprot.org/core/author | "Matsushita K."xsd:string |
| http://purl.uniprot.org/citations/20080593 | http://purl.uniprot.org/core/author | "Matsushita K."xsd:string |
| http://purl.uniprot.org/citations/20080593 | http://purl.uniprot.org/core/author | "Fujita T."xsd:string |
| http://purl.uniprot.org/citations/20080593 | http://purl.uniprot.org/core/author | "Fujita T."xsd:string |
| http://purl.uniprot.org/citations/20080593 | http://purl.uniprot.org/core/author | "Sato S."xsd:string |
| http://purl.uniprot.org/citations/20080593 | http://purl.uniprot.org/core/author | "Sato S."xsd:string |
| http://purl.uniprot.org/citations/20080593 | http://purl.uniprot.org/core/author | "Satoh T."xsd:string |
| http://purl.uniprot.org/citations/20080593 | http://purl.uniprot.org/core/author | "Satoh T."xsd:string |
| http://purl.uniprot.org/citations/20080593 | http://purl.uniprot.org/core/author | "Akira S."xsd:string |
| http://purl.uniprot.org/citations/20080593 | http://purl.uniprot.org/core/author | "Akira S."xsd:string |
| http://purl.uniprot.org/citations/20080593 | http://purl.uniprot.org/core/author | "Tsujimura T."xsd:string |
| http://purl.uniprot.org/citations/20080593 | http://purl.uniprot.org/core/author | "Tsujimura T."xsd:string |
| http://purl.uniprot.org/citations/20080593 | http://purl.uniprot.org/core/author | "Takeuchi O."xsd:string |
| http://purl.uniprot.org/citations/20080593 | http://purl.uniprot.org/core/author | "Takeuchi O."xsd:string |
| http://purl.uniprot.org/citations/20080593 | http://purl.uniprot.org/core/author | "Yoneyama M."xsd:string |
| http://purl.uniprot.org/citations/20080593 | http://purl.uniprot.org/core/author | "Yoneyama M."xsd:string |
| http://purl.uniprot.org/citations/20080593 | http://purl.uniprot.org/core/author | "Kumagai Y."xsd:string |
| http://purl.uniprot.org/citations/20080593 | http://purl.uniprot.org/core/author | "Kumagai Y."xsd:string |