| http://purl.uniprot.org/citations/20097930 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20097930 | http://www.w3.org/2000/01/rdf-schema#comment | "We have shown that expression of apolipoprotein (apo) C-III promotes VLDL secretion from transfected McA-RH7777 cells under lipid-rich conditions. To determine structural elements within apoC-III that confer to this function, we contrasted wild-type apoC-III with a mutant Ala23Thr originally identified in hypotriglyceridemia subjects. Although synthesis of [(3)H]glycerol-labeled TAG was comparable between cells expressing wild-type apoC-III (C3wt cells) or Ala23Thr mutant (C3AT cells), secretion of [(3)H]TAG from C3AT cells was markedly decreased. The lowered [(3)H]TAG secretion was associated with an inability of C3AT cells to assemble VLDL(1). Moreover, [(3)H]TAG within the microsomal lumen in C3AT cells was 60% higher than that in C3wt cells, yet the activity of microsomal triglyceride-transfer protein in C3AT cells was not elevated. The accumulated [(3)H]TAG in C3AT microsomal lumen was mainly associated with lumenal IDL/LDL-like lipoproteins. Phenotypically, this [(3)H]TAG fractionation profiling resembled what was observed in cells treated with brefeldin A, which at low dose specifically blocked the second-step VLDL(1) maturation. Furthermore, lumenal [(35)S]Ala23Thr protein accumulated in IDL/LDL fractions and was absent in VLDL fractions in C3AT cells. These results suggest that the presence of Ala23Thr protein in lumenal IDL/LDL particles might prevent effective fusion between lipid droplets and VLDL precursors. Thus, the current study reveals an important structural element residing within the N-terminal region of apoC-III that governs the second step VLDL(1) maturation."xsd:string |
| http://purl.uniprot.org/citations/20097930 | http://purl.org/dc/terms/identifier | "doi:10.1194/jlr.m005108"xsd:string |
| http://purl.uniprot.org/citations/20097930 | http://purl.uniprot.org/core/author | "Sundaram M."xsd:string |
| http://purl.uniprot.org/citations/20097930 | http://purl.uniprot.org/core/author | "Wang Y."xsd:string |
| http://purl.uniprot.org/citations/20097930 | http://purl.uniprot.org/core/author | "Zhou H."xsd:string |
| http://purl.uniprot.org/citations/20097930 | http://purl.uniprot.org/core/author | "Zhao Y."xsd:string |
| http://purl.uniprot.org/citations/20097930 | http://purl.uniprot.org/core/author | "Yao Z."xsd:string |
| http://purl.uniprot.org/citations/20097930 | http://purl.uniprot.org/core/author | "Zhong S."xsd:string |
| http://purl.uniprot.org/citations/20097930 | http://purl.uniprot.org/core/author | "Figeys D."xsd:string |
| http://purl.uniprot.org/citations/20097930 | http://purl.uniprot.org/core/author | "Hussain M.M."xsd:string |
| http://purl.uniprot.org/citations/20097930 | http://purl.uniprot.org/core/author | "Iqbal J."xsd:string |
| http://purl.uniprot.org/citations/20097930 | http://purl.uniprot.org/core/author | "Jiang Z.G."xsd:string |
| http://purl.uniprot.org/citations/20097930 | http://purl.uniprot.org/core/author | "Bou Khalil M."xsd:string |
| http://purl.uniprot.org/citations/20097930 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20097930 | http://purl.uniprot.org/core/name | "J Lipid Res"xsd:string |
| http://purl.uniprot.org/citations/20097930 | http://purl.uniprot.org/core/pages | "1524-1534"xsd:string |
| http://purl.uniprot.org/citations/20097930 | http://purl.uniprot.org/core/title | "Functional analysis of the missense APOC3 mutation Ala23Thr associated with human hypotriglyceridemia."xsd:string |
| http://purl.uniprot.org/citations/20097930 | http://purl.uniprot.org/core/volume | "51"xsd:string |
| http://purl.uniprot.org/citations/20097930 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20097930 |
| http://purl.uniprot.org/citations/20097930 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20097930 |
| http://purl.uniprot.org/uniprot/#_A3KPE2-mappedCitation-20097930 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20097930 |
| http://purl.uniprot.org/uniprot/#_B0YIW2-mappedCitation-20097930 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20097930 |
| http://purl.uniprot.org/uniprot/#_P02656-mappedCitation-20097930 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20097930 |
| http://purl.uniprot.org/uniprot/A3KPE2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20097930 |