| http://purl.uniprot.org/citations/20107186 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20107186 | http://www.w3.org/2000/01/rdf-schema#comment | "Transient receptor potential melastatin 2 (TRPM2) is a Ca(2+)-permeable nonselective cation channel that is stimulated by oxidative stress and specifically activated by intracellular ADP-ribose. Because TRPM2 is highly expressed in immunocytes, a role of this channel in inflammation processes has been proposed. The aim of the current study was to determine the function of TRPM2 in LPS-induced cytokine production of human monocytes. Incubation of human primary monocytes with LPS resulted in an upregulation of TRPM2 mRNA, protein, and of ADP-ribose-induced membrane currents. By using short hairpin RNA to downregulate TRPM2 expression in THP-1 monocytes, we demonstrate that TRPM2 is required for the LPS-induced production of IL-6, IL-8, IL-10, and TNF-alpha. Application of LPS led to a time-dependent increase in intracellular Ca(2+) concentrations in THP-1 cells that was clearly reduced by downregulation of TRPM2. Omission of extracellular Ca(2+) strongly decreased TNF-alpha production in TRPM2-expressing cells. Thus, TRPM2-mediated Ca(2+) entry is a central mechanism for LPS-induced cytokine production in monocytic cells. The identification of TRPM2 as a major player in this LPS-dependent process makes it a promising tool in modulating monocyte functions."xsd:string |
| http://purl.uniprot.org/citations/20107186 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.0902474"xsd:string |
| http://purl.uniprot.org/citations/20107186 | http://purl.uniprot.org/core/author | "Kraft R."xsd:string |
| http://purl.uniprot.org/citations/20107186 | http://purl.uniprot.org/core/author | "Harteneck C."xsd:string |
| http://purl.uniprot.org/citations/20107186 | http://purl.uniprot.org/core/author | "Hauschildt S."xsd:string |
| http://purl.uniprot.org/citations/20107186 | http://purl.uniprot.org/core/author | "Wehrhahn J."xsd:string |
| http://purl.uniprot.org/citations/20107186 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20107186 | http://purl.uniprot.org/core/name | "J Immunol"xsd:string |
| http://purl.uniprot.org/citations/20107186 | http://purl.uniprot.org/core/pages | "2386-2393"xsd:string |
| http://purl.uniprot.org/citations/20107186 | http://purl.uniprot.org/core/title | "Transient receptor potential melastatin 2 is required for lipopolysaccharide-induced cytokine production in human monocytes."xsd:string |
| http://purl.uniprot.org/citations/20107186 | http://purl.uniprot.org/core/volume | "184"xsd:string |
| http://purl.uniprot.org/citations/20107186 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20107186 |
| http://purl.uniprot.org/citations/20107186 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20107186 |
| http://purl.uniprot.org/uniprot/#_A0A0G2YPN5-mappedCitation-20107186 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20107186 |
| http://purl.uniprot.org/uniprot/#_A0A1U9X8M9-mappedCitation-20107186 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20107186 |
| http://purl.uniprot.org/uniprot/#_A0A411D318-mappedCitation-20107186 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20107186 |
| http://purl.uniprot.org/uniprot/#_A0A7U3S0Q4-mappedCitation-20107186 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20107186 |
| http://purl.uniprot.org/uniprot/#_A0A1C9J7U0-mappedCitation-20107186 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20107186 |
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| http://purl.uniprot.org/uniprot/#_B4DVM1-mappedCitation-20107186 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20107186 |
| http://purl.uniprot.org/uniprot/#_B5BUQ6-mappedCitation-20107186 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20107186 |
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| http://purl.uniprot.org/uniprot/#_B4DNV3-mappedCitation-20107186 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20107186 |