http://purl.uniprot.org/citations/20128730 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/20128730 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundThe identification of human mitochondrial DNA (mtDNA) sequence variations, especially single nucleotide polymorphisms (SNPs), is important for many applications. The PCR-ligase detection reaction (LDR) method can reduce false-positives and eliminate the need for both post-PCR and post-ligation purifications in SNP analyses. In addition, it has been successfully employed to detect point mutations in various nuclear genes. In this study, we used the PCR-LDR platform to characterize mtDNA SNPs.MethodsMultiplex PCR-LDRs were used to genotype 19 mtDNA single nucleotide polymorphic sites from 812 samples. Performance of the method was assessed by direct sequencing of 44 samples.ResultsWe established an overall 97.4% success rate with 99.2% accuracy using the multiplex PCR-LDR methodology.ConclusionsThe PCR-LDR mtDNA genotyping technique is simple, highly accurate, has high-throughput, and is cost-effective. Therefore, this method is applicable to mtDNA haplotyping in various applications."xsd:string |
http://purl.uniprot.org/citations/20128730 | http://purl.org/dc/terms/identifier | "doi:10.1515/cclm.2010.097"xsd:string |
http://purl.uniprot.org/citations/20128730 | http://purl.uniprot.org/core/author | "Chen L."xsd:string |
http://purl.uniprot.org/citations/20128730 | http://purl.uniprot.org/core/author | "Gao Y."xsd:string |
http://purl.uniprot.org/citations/20128730 | http://purl.uniprot.org/core/author | "Gao W."xsd:string |
http://purl.uniprot.org/citations/20128730 | http://purl.uniprot.org/core/author | "Luo Y."xsd:string |
http://purl.uniprot.org/citations/20128730 | http://purl.uniprot.org/core/author | "Huang T."xsd:string |
http://purl.uniprot.org/citations/20128730 | http://purl.uniprot.org/core/author | "Yang X."xsd:string |
http://purl.uniprot.org/citations/20128730 | http://purl.uniprot.org/core/author | "Tang S."xsd:string |
http://purl.uniprot.org/citations/20128730 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
http://purl.uniprot.org/citations/20128730 | http://purl.uniprot.org/core/name | "Clin Chem Lab Med"xsd:string |
http://purl.uniprot.org/citations/20128730 | http://purl.uniprot.org/core/pages | "475-483"xsd:string |
http://purl.uniprot.org/citations/20128730 | http://purl.uniprot.org/core/title | "Genotyping mitochondrial DNA single nucleotide polymorphisms by PCR ligase detection reactions."xsd:string |
http://purl.uniprot.org/citations/20128730 | http://purl.uniprot.org/core/volume | "48"xsd:string |
http://purl.uniprot.org/citations/20128730 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20128730 |
http://purl.uniprot.org/citations/20128730 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20128730 |
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http://purl.uniprot.org/uniprot/#_B4F436-mappedCitation-20128730 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20128730 |