| http://purl.uniprot.org/citations/2013320 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2013320 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2013320 | http://www.w3.org/2000/01/rdf-schema#comment | "Antithrombin III Hamilton is a structural variant of antithrombin III (AT-III) with normal heparin affinity but impaired serine protease inhibitory activity. The molecular defect of AT-III-Hamilton is a substitution of threonine for alanine at amino acid residue 382. Recently it has been shown that both plasma-derived and cell-free-derived AT-III-Hamilton polypeptides act as substrates rather than inhibitors of thrombin and factor Xa. In the present study, the cell-free expression phagemid vector pGEM-3Zf(+)-AT-III1-432 was mutated at amino acid residue 382 of AT-III to generate 7 cell-free-derived variants. All these cell-free-derived AT-III variants were able to bind heparin as effectively as cell-free-derived normal AT-III. In terms of alpha-thrombin inhibitory activity each variant reacted differently. Variants could be grouped into 3 categories with respect to thrombin-AT-III complex formation: (1) near normal activity (glycine, isoleucine, leucine, valine); (2) low activity (threonine, glutamine); (3) no detectable activity (lysine). These data suggest that mutations at position 382 of AT-III may have a variable effect on protease inhibitory activity, depending on either the stability of the P12-P9 region of the exposed loop of AT-III, or the inability of the amino acid residue at position 382 to interact with a conserved hydrophobic pocket consisting of phenylalanine (at positions 77, 221 and 422) and isoleucine (position 412) residues."xsd:string |
| http://purl.uniprot.org/citations/2013320 | http://purl.org/dc/terms/identifier | "doi:10.1016/0014-5793(91)80305-m"xsd:string |
| http://purl.uniprot.org/citations/2013320 | http://purl.org/dc/terms/identifier | "doi:10.1016/0014-5793(91)80305-m"xsd:string |
| http://purl.uniprot.org/citations/2013320 | http://purl.uniprot.org/core/author | "Rachubinski R.A."xsd:string |
| http://purl.uniprot.org/citations/2013320 | http://purl.uniprot.org/core/author | "Rachubinski R.A."xsd:string |
| http://purl.uniprot.org/citations/2013320 | http://purl.uniprot.org/core/author | "Austin R.C."xsd:string |
| http://purl.uniprot.org/citations/2013320 | http://purl.uniprot.org/core/author | "Austin R.C."xsd:string |
| http://purl.uniprot.org/citations/2013320 | http://purl.uniprot.org/core/author | "Blachjman M.A."xsd:string |
| http://purl.uniprot.org/citations/2013320 | http://purl.uniprot.org/core/author | "Blachjman M.A."xsd:string |
| http://purl.uniprot.org/citations/2013320 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/2013320 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/2013320 | http://purl.uniprot.org/core/name | "FEBS Lett."xsd:string |
| http://purl.uniprot.org/citations/2013320 | http://purl.uniprot.org/core/name | "FEBS Lett."xsd:string |
| http://purl.uniprot.org/citations/2013320 | http://purl.uniprot.org/core/pages | "254-258"xsd:string |
| http://purl.uniprot.org/citations/2013320 | http://purl.uniprot.org/core/pages | "254-258"xsd:string |
| http://purl.uniprot.org/citations/2013320 | http://purl.uniprot.org/core/title | "Site-directed mutagenesis of alanine-382 of human antithrombin III."xsd:string |
| http://purl.uniprot.org/citations/2013320 | http://purl.uniprot.org/core/title | "Site-directed mutagenesis of alanine-382 of human antithrombin III."xsd:string |
| http://purl.uniprot.org/citations/2013320 | http://purl.uniprot.org/core/volume | "280"xsd:string |
| http://purl.uniprot.org/citations/2013320 | http://purl.uniprot.org/core/volume | "280"xsd:string |
| http://purl.uniprot.org/citations/2013320 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2013320 |
| http://purl.uniprot.org/citations/2013320 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2013320 |
| http://purl.uniprot.org/citations/2013320 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/2013320 |
| http://purl.uniprot.org/citations/2013320 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/2013320 |