| http://purl.uniprot.org/citations/20133939 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20133939 | http://www.w3.org/2000/01/rdf-schema#comment | "The alpha subunit of stimulatory G protein (G alpha(s)) activates adenylyl cyclase, which catalyzes cAMP production, and regulates many physiological aspects, such as cardiac regulation and endocrine systems. Ric-8B (resistance to inhibitors of cholinesterase 8B) has been identified as the G alpha(s)-binding protein; however, its role in G(s) signaling remains obscure. In this study, we present evidence that Ric-8B specifically and positively regulates G(s) signaling by stabilizing the G alpha(s) protein. An in vitro biochemical study suggested that Ric-8B does not possess guanine nucleotide exchange factor activity. However, knockdown of Ric-8B attenuated beta-adrenergic agonist-induced cAMP accumulation, indicating that Ric-8B positively regulates G(s) signaling. Interestingly, overexpression and knockdown of Ric-8B resulted in an increase and a decrease in the G alpha(s) protein, respectively, without affecting the G alpha(s) mRNA level. We found that the G alpha(s) protein is ubiquitinated and that this ubiquitination is inhibited by Ric-8B. This Ric-8B-mediated inhibition of G alpha(s) ubiquitination requires interaction between Ric-8B and G alpha(s) because Ric-8B splicing variants, which are defective for G alpha(s) binding, failed to inhibit the ubiquitination. Taken together, these results suggest that Ric-8B plays a critical and specific role in the control of G alpha(s) protein levels by modulating G alpha(s) ubiquitination and positively regulates G(s) signaling."xsd:string |
| http://purl.uniprot.org/citations/20133939 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m109.063313"xsd:string |
| http://purl.uniprot.org/citations/20133939 | http://purl.uniprot.org/core/author | "Nagai Y."xsd:string |
| http://purl.uniprot.org/citations/20133939 | http://purl.uniprot.org/core/author | "Itoh H."xsd:string |
| http://purl.uniprot.org/citations/20133939 | http://purl.uniprot.org/core/author | "Nishimura A."xsd:string |
| http://purl.uniprot.org/citations/20133939 | http://purl.uniprot.org/core/author | "Mizuno N."xsd:string |
| http://purl.uniprot.org/citations/20133939 | http://purl.uniprot.org/core/author | "Tago K."xsd:string |
| http://purl.uniprot.org/citations/20133939 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20133939 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/20133939 | http://purl.uniprot.org/core/pages | "11114-11120"xsd:string |
| http://purl.uniprot.org/citations/20133939 | http://purl.uniprot.org/core/title | "Ric-8B stabilizes the alpha subunit of stimulatory G protein by inhibiting its ubiquitination."xsd:string |
| http://purl.uniprot.org/citations/20133939 | http://purl.uniprot.org/core/volume | "285"xsd:string |
| http://purl.uniprot.org/citations/20133939 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20133939 |
| http://purl.uniprot.org/citations/20133939 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20133939 |
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| http://purl.uniprot.org/uniprot/#_A0A0S2Z3S5-mappedCitation-20133939 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20133939 |
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