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http://purl.uniprot.org/citations/20134267http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20134267http://www.w3.org/2000/01/rdf-schema#comment"

Objectives

High-risk human papillomaviruses (HPVs) are the major causative agents of cervical cancer, and the E6 and E7 genes encode the major HPV oncoproteins. The E7 protein of high-risk HPV types disturbs cell cycle control and down-regulates components of the antigen presentation pathway, suggesting a role for E7 in tumor immune evasion. We previously reported that HPV-16 E7 expression and down-regulation of HLA class I was highly correlated in cervical lesions. This study was aimed to determine whether HPV-16 E7 oncoprotein could down-regulate surface HLA class I antigen in HPV-16 E7-transfected cells, and whether it had correlation with the expression of the transporter associated with antigen processing (TAP).

Methods

The HPV-16 E7 open reading frame was transfected into HaCaT cells. After G418 selection, resistant colonies were individually picked and expanded into clonal cell lines. Using the fluoresence-activated cell sorting analysis, the levels of cell surface HLA class I antigen and intracellular TAP-1 and TAP-2 expressions were detected.

Results

Compared with the empty vector control, a statistical significant decrease of approximately 50% in cell surface HLA class I expression was observed in HPV-16 E7 expressing HaCaT cells (P < 0.001). Moreover, the expression of HPV-16 E7 in HaCaT cells resulted in decreased expression of TAP-1 that was essential for HLA class I expression at the cell surface, a statistical significant decrease of approximately 40% compared with that with the empty vector control (P < 0.001).

Conclusions

Our finding demonstrates that HPV-16 E7 down-regulates surface HLA class I antigen, which in part correlates with the decrease of TAP-1."xsd:string
http://purl.uniprot.org/citations/20134267http://purl.org/dc/terms/identifier"doi:10.1111/igc.0b013e3181cceec5"xsd:string
http://purl.uniprot.org/citations/20134267http://purl.uniprot.org/core/author"Li W."xsd:string
http://purl.uniprot.org/citations/20134267http://purl.uniprot.org/core/author"Zhang X."xsd:string
http://purl.uniprot.org/citations/20134267http://purl.uniprot.org/core/author"Zhang J."xsd:string
http://purl.uniprot.org/citations/20134267http://purl.uniprot.org/core/author"Wang C.X."xsd:string
http://purl.uniprot.org/citations/20134267http://purl.uniprot.org/core/author"Zheng G.X."xsd:string
http://purl.uniprot.org/citations/20134267http://purl.uniprot.org/core/author"Deng X.M."xsd:string
http://purl.uniprot.org/citations/20134267http://purl.uniprot.org/core/author"Feng J.B."xsd:string
http://purl.uniprot.org/citations/20134267http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/20134267http://purl.uniprot.org/core/name"Int J Gynecol Cancer"xsd:string
http://purl.uniprot.org/citations/20134267http://purl.uniprot.org/core/pages"227-232"xsd:string
http://purl.uniprot.org/citations/20134267http://purl.uniprot.org/core/title"Down-regulation of HLA class I antigen in human papillomavirus type 16 E7 expressing HaCaT cells: correlate with TAP-1 expression."xsd:string
http://purl.uniprot.org/citations/20134267http://purl.uniprot.org/core/volume"20"xsd:string
http://purl.uniprot.org/citations/20134267http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/20134267
http://purl.uniprot.org/citations/20134267http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/20134267
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