| http://purl.uniprot.org/citations/20139075 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20139075 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20139075 | http://www.w3.org/2000/01/rdf-schema#comment | "Germ line mutations of the ubiquitin ligase cullin 7 (CUL7) are linked to 3-M syndrome and Yakuts short stature syndrome, both of which are characterized by pre- and post-natal growth retardation. CUL7 knock-out mice show placental and embryonic defects similar to intrauterine growth retardation, suggesting a role of CUL7 in placentation. CUL7 was found in this study to be highly expressed in first trimester invasive human placental villi as well as in HTR8/SVneo and B6Tert cells, two cell lines derived from human first trimester trophoblast cells. However, CUL7 levels in term trophoblast cells or JEG-3 cells, which are derived from human choriocarcinoma but exhibit weak invasion capacity, were low or undetectable. Forced expression of CUL7 in JEG-3 cells induced cell morphological changes characteristic of epithelial-mesenchymal transition, which was accompanied by a complete loss of the epithelial markers E-cadherin and P-cadherin and a significant elevation of mesenchymal markers Vimentin and N-cadherin. JEG-3 cells expressing CUL7 exhibited enhanced cell migration and invasion. Conversely, CUL7-specific RNA interference in HTR8/SVneo cells resulted in increased E-cadherin expression and reduced cell migration and invasion. Furthermore, CUL7 expression down-regulated E-cadherin mRNA expression by up-regulating ZEB1 and Slug, two transcriptional repressors of E-cadherin. Finally, CUL7-induced loss of E-cadherin expression was partially reversed by treatment of CUL7-expressing cells with the proteasome inhibitor MG-132. These results suggest that the CUL7 E3 ligase is a key regulator in trophoblast cell epithelial-mesenchymal transition and placental development."xsd:string |
| http://purl.uniprot.org/citations/20139075 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m109.004200"xsd:string |
| http://purl.uniprot.org/citations/20139075 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m109.004200"xsd:string |
| http://purl.uniprot.org/citations/20139075 | http://purl.uniprot.org/core/author | "Lin H."xsd:string |
| http://purl.uniprot.org/citations/20139075 | http://purl.uniprot.org/core/author | "Lin H."xsd:string |
| http://purl.uniprot.org/citations/20139075 | http://purl.uniprot.org/core/author | "Zhou Z."xsd:string |
| http://purl.uniprot.org/citations/20139075 | http://purl.uniprot.org/core/author | "Zhou Z."xsd:string |
| http://purl.uniprot.org/citations/20139075 | http://purl.uniprot.org/core/author | "Wang H."xsd:string |
| http://purl.uniprot.org/citations/20139075 | http://purl.uniprot.org/core/author | "Wang H."xsd:string |
| http://purl.uniprot.org/citations/20139075 | http://purl.uniprot.org/core/author | "Wu L."xsd:string |
| http://purl.uniprot.org/citations/20139075 | http://purl.uniprot.org/core/author | "Wu L."xsd:string |
| http://purl.uniprot.org/citations/20139075 | http://purl.uniprot.org/core/author | "Zhang B."xsd:string |
| http://purl.uniprot.org/citations/20139075 | http://purl.uniprot.org/core/author | "Zhang B."xsd:string |
| http://purl.uniprot.org/citations/20139075 | http://purl.uniprot.org/core/author | "Wang R."xsd:string |
| http://purl.uniprot.org/citations/20139075 | http://purl.uniprot.org/core/author | "Wang R."xsd:string |
| http://purl.uniprot.org/citations/20139075 | http://purl.uniprot.org/core/author | "Fu J."xsd:string |
| http://purl.uniprot.org/citations/20139075 | http://purl.uniprot.org/core/author | "Fu J."xsd:string |
| http://purl.uniprot.org/citations/20139075 | http://purl.uniprot.org/core/author | "Zhu C."xsd:string |
| http://purl.uniprot.org/citations/20139075 | http://purl.uniprot.org/core/author | "Zhu C."xsd:string |
| http://purl.uniprot.org/citations/20139075 | http://purl.uniprot.org/core/author | "Wang Y.L."xsd:string |
| http://purl.uniprot.org/citations/20139075 | http://purl.uniprot.org/core/author | "Wang Y.L."xsd:string |
| http://purl.uniprot.org/citations/20139075 | http://purl.uniprot.org/core/author | "Tsang B.K."xsd:string |
| http://purl.uniprot.org/citations/20139075 | http://purl.uniprot.org/core/author | "Tsang B.K."xsd:string |