http://purl.uniprot.org/citations/20140636 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/20140636 | http://www.w3.org/2000/01/rdf-schema#comment | "PurposeHuman organic anion transporter 4 (hOAT4) belongs to a family of organic anion transporters that play critical roles in the body disposition of clinically important drugs. We have previously shown that the activity of hOAT4 was down-regulated by activation of PKC and up-regulated by PDZ protein NHERF-1. Here, we investigated the mechanisms underlying such regulations.MethodsCOS-7 cells expressing hOAT4 were treated with PKC activator phorbol 12-myristate 13-acetate (PMA) or transfected with dominant negative mutants of dynamin-2 or Eps15 or transfected with NHERF-1. The internalization and the function of hOAT4 were then determined.ResultsWe showed that hOAT4 constitutively internalized from and recycled back to plasma membrane. Transfection of dominant negative mutants of dynamin-2 or Eps15 into the cells, all of which block clathrin-dependent endocytotic pathway, significantly blocked hOAT4 internalization. Treatment of cells with PMA accelerated hOAT4 internalization, whereas transfection of cells with NHERF-1 attenuated hOAT4 internalization.ConclusionOur studies demonstrated that i) hOAT4 undergoes constitutive trafficking between cell surface and intracellular compartments, ii) hOAT4 internalization partly occurs through clathrin-dependent pathway, iii) the down-regulation of hOAT4 activity by activation of PKC and the up-regulation of hOAT4 activity by NHERF-1 are mediated through alteration of hOAT4 internalization."xsd:string |
http://purl.uniprot.org/citations/20140636 | http://purl.org/dc/terms/identifier | "doi:10.1007/s11095-009-9983-2"xsd:string |
http://purl.uniprot.org/citations/20140636 | http://purl.uniprot.org/core/author | "Zhang Q."xsd:string |
http://purl.uniprot.org/citations/20140636 | http://purl.uniprot.org/core/author | "Pan Z."xsd:string |
http://purl.uniprot.org/citations/20140636 | http://purl.uniprot.org/core/author | "You G."xsd:string |
http://purl.uniprot.org/citations/20140636 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
http://purl.uniprot.org/citations/20140636 | http://purl.uniprot.org/core/name | "Pharm Res"xsd:string |
http://purl.uniprot.org/citations/20140636 | http://purl.uniprot.org/core/pages | "589-596"xsd:string |
http://purl.uniprot.org/citations/20140636 | http://purl.uniprot.org/core/title | "Regulation of human organic anion transporter 4 by protein kinase C and NHERF-1: altering the endocytosis of the transporter."xsd:string |
http://purl.uniprot.org/citations/20140636 | http://purl.uniprot.org/core/volume | "27"xsd:string |
http://purl.uniprot.org/citations/20140636 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20140636 |
http://purl.uniprot.org/citations/20140636 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20140636 |
http://purl.uniprot.org/uniprot/#_B3KSV9-mappedCitation-20140636 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20140636 |
http://purl.uniprot.org/uniprot/#_B2R6A3-mappedCitation-20140636 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20140636 |
http://purl.uniprot.org/uniprot/#_B4DJH6-mappedCitation-20140636 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20140636 |
http://purl.uniprot.org/uniprot/#_O14745-mappedCitation-20140636 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20140636 |
http://purl.uniprot.org/uniprot/#_Q9NSA0-mappedCitation-20140636 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20140636 |
http://purl.uniprot.org/uniprot/#_Q8NBZ3-mappedCitation-20140636 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20140636 |
http://purl.uniprot.org/uniprot/O14745 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20140636 |
http://purl.uniprot.org/uniprot/B2R6A3 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20140636 |
http://purl.uniprot.org/uniprot/B4DJH6 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20140636 |
http://purl.uniprot.org/uniprot/B3KSV9 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20140636 |
http://purl.uniprot.org/uniprot/Q9NSA0 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20140636 |
http://purl.uniprot.org/uniprot/Q8NBZ3 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20140636 |