| http://purl.uniprot.org/citations/20144232 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20144232 | http://www.w3.org/2000/01/rdf-schema#comment | "SIAH proteins are the human members of an highly conserved family of E3 ubiquitin ligases. Several data suggest that SIAH proteins may have a role in tumor suppression and apoptosis. Previously, we reported that SIAH-1 induces the degradation of Kid (KIF22), a chromokinesin protein implicated in the normal progression of mitosis and meiosis, by the ubiquitin proteasome pathway. In human breast cancer cells stably transfected with SIAH-1, Kid/KIF22 protein level was markedly reduced whereas, the Kid/KIF22 mRNA level was increased. This interaction has been further elucidated through analyzing SIAH and Kid/KIF22 expression in both paired normal and tumor tissues and cell lines. It was observed that SIAH-1 protein is widely expressed in different normal tissues, and in cells lines but showing some differences in western blotting profiles. Immunofluorescence microscopy shows that the intracellular distribution of SIAH-1 and Kid/KIF22 appears to be modified in human tumor tissues compared to normal controls. When mRNA expression of SIAH-1 and Kid/KIF22 was analyzed by real-time PCR in normal and cancer breast tissues from the same patient, a large variation in the number of mRNA copies was detected between the different samples. In most cases, SIAH-1 mRNA is decreased in tumor tissues compared to their normal counterparts. Interestingly, in all breast tumor tissues analyzed, variations in the Kid/KIF22 mRNA levels mirrored those seen with SIAH-1 mRNAs. This concerted variation of SIAH-1 and Kid/KIF22 messengers suggests the existence of an additional level of control than the previously described protein-protein interaction and protein stability regulation. Our observations also underline the need to re-evaluate the results of gene expression obtained by qRT-PCR and relate it to the protein expression and cellular localization when matched normal and tumoral tissues are analyzed."xsd:string |
| http://purl.uniprot.org/citations/20144232 | http://purl.org/dc/terms/identifier | "doi:10.1186/1756-9966-29-10"xsd:string |
| http://purl.uniprot.org/citations/20144232 | http://purl.uniprot.org/core/author | "Fernandez P."xsd:string |
| http://purl.uniprot.org/citations/20144232 | http://purl.uniprot.org/core/author | "Powell D.J."xsd:string |
| http://purl.uniprot.org/citations/20144232 | http://purl.uniprot.org/core/author | "Marin M."xsd:string |
| http://purl.uniprot.org/citations/20144232 | http://purl.uniprot.org/core/author | "Calvo F."xsd:string |
| http://purl.uniprot.org/citations/20144232 | http://purl.uniprot.org/core/author | "Mourah S."xsd:string |
| http://purl.uniprot.org/citations/20144232 | http://purl.uniprot.org/core/author | "Podgorniak M.P."xsd:string |
| http://purl.uniprot.org/citations/20144232 | http://purl.uniprot.org/core/author | "Bruzzoni-Giovanelli H."xsd:string |
| http://purl.uniprot.org/citations/20144232 | http://purl.uniprot.org/core/author | "Candeias M.M."xsd:string |
| http://purl.uniprot.org/citations/20144232 | http://purl.uniprot.org/core/author | "Veiga L."xsd:string |
| http://purl.uniprot.org/citations/20144232 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20144232 | http://purl.uniprot.org/core/name | "J Exp Clin Cancer Res"xsd:string |
| http://purl.uniprot.org/citations/20144232 | http://purl.uniprot.org/core/pages | "10"xsd:string |
| http://purl.uniprot.org/citations/20144232 | http://purl.uniprot.org/core/title | "Distinct expression patterns of the E3 ligase SIAH-1 and its partner Kid/KIF22 in normal tissues and in the breast tumoral processes."xsd:string |
| http://purl.uniprot.org/citations/20144232 | http://purl.uniprot.org/core/volume | "29"xsd:string |
| http://purl.uniprot.org/citations/20144232 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20144232 |
| http://purl.uniprot.org/citations/20144232 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20144232 |
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| http://purl.uniprot.org/uniprot/#_O60846-mappedCitation-20144232 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20144232 |