| http://purl.uniprot.org/citations/20164179 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20164179 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20164179 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/20164179 | http://www.w3.org/2000/01/rdf-schema#comment | "Selenocysteine lyase (SCL) catalyzes the pyridoxal 5'-phosphate-dependent removal of selenium from l-selenocysteine to yield l-alanine. The enzyme is proposed to function in the recycling of the micronutrient selenium from degraded selenoproteins containing selenocysteine residue as an essential component. The enzyme exhibits strict substrate specificity toward l-selenocysteine and no activity to its cognate l-cysteine. However, it remains unclear how the enzyme distinguishes between selenocysteine and cysteine. Here, we present mechanistic studies of selenocysteine lyase from rat. ESI-MS analysis of wild-type and C375A mutant SCL revealed that the catalytic reaction proceeds via the formation of an enzyme-bound selenopersulfide intermediate on the catalytically essential Cys-375 residue. UV-visible spectrum analysis and the crystal structure of SCL complexed with l-cysteine demonstrated that the enzyme reversibly forms a nonproductive adduct with l-cysteine. Cys-375 on the flexible loop directed l-selenocysteine, but not l-cysteine, to the correct position and orientation in the active site to initiate the catalytic reaction. These findings provide, for the first time, the basis for understanding how trace amounts of a selenium-containing substrate is distinguished from excessive amounts of its cognate sulfur-containing compound in a biological system."xsd:string |
| http://purl.uniprot.org/citations/20164179 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m109.084475"xsd:string |
| http://purl.uniprot.org/citations/20164179 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m109.084475"xsd:string |
| http://purl.uniprot.org/citations/20164179 | http://purl.uniprot.org/core/author | "Hayashi H."xsd:string |
| http://purl.uniprot.org/citations/20164179 | http://purl.uniprot.org/core/author | "Hayashi H."xsd:string |
| http://purl.uniprot.org/citations/20164179 | http://purl.uniprot.org/core/author | "Hirotsu K."xsd:string |
| http://purl.uniprot.org/citations/20164179 | http://purl.uniprot.org/core/author | "Hirotsu K."xsd:string |
| http://purl.uniprot.org/citations/20164179 | http://purl.uniprot.org/core/author | "Miyahara I."xsd:string |
| http://purl.uniprot.org/citations/20164179 | http://purl.uniprot.org/core/author | "Miyahara I."xsd:string |
| http://purl.uniprot.org/citations/20164179 | http://purl.uniprot.org/core/author | "Esaki N."xsd:string |
| http://purl.uniprot.org/citations/20164179 | http://purl.uniprot.org/core/author | "Esaki N."xsd:string |
| http://purl.uniprot.org/citations/20164179 | http://purl.uniprot.org/core/author | "Kurihara T."xsd:string |
| http://purl.uniprot.org/citations/20164179 | http://purl.uniprot.org/core/author | "Kurihara T."xsd:string |
| http://purl.uniprot.org/citations/20164179 | http://purl.uniprot.org/core/author | "Goto M."xsd:string |
| http://purl.uniprot.org/citations/20164179 | http://purl.uniprot.org/core/author | "Goto M."xsd:string |
| http://purl.uniprot.org/citations/20164179 | http://purl.uniprot.org/core/author | "Kurokawa S."xsd:string |
| http://purl.uniprot.org/citations/20164179 | http://purl.uniprot.org/core/author | "Kurokawa S."xsd:string |
| http://purl.uniprot.org/citations/20164179 | http://purl.uniprot.org/core/author | "Mihara H."xsd:string |
| http://purl.uniprot.org/citations/20164179 | http://purl.uniprot.org/core/author | "Mihara H."xsd:string |
| http://purl.uniprot.org/citations/20164179 | http://purl.uniprot.org/core/author | "Omi R."xsd:string |
| http://purl.uniprot.org/citations/20164179 | http://purl.uniprot.org/core/author | "Omi R."xsd:string |
| http://purl.uniprot.org/citations/20164179 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |