| http://purl.uniprot.org/citations/20172014 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20172014 | http://www.w3.org/2000/01/rdf-schema#comment | "Le(Y) antigen is known to be associated with malignant properties including metastasis and a poor prognosis of ovarian carcinomas. To clarify the mechanisms underling these properties, we established ovarian carcinoma-derived cells exhibiting enhanced expression of Le(Y) by transfection with alpha1,2-fucosyltransferase and compared their cellular properties with those of the original cells. So the human alpha1,2-fucosyltransferase gene was transfected into ovarian carcinoma-derived RMG-1 cells, which are known to contain Le(X), a precursor of Le(Y), and RMG-1-hFUT cells exhibiting enhanced expression of Le(Y) were established by selection with anti-Le(Y) antibodies, and their adhesive and spreading potentials on fibronectin-coated plates were compared with those of RMG-1 cells. Results showed that the relative expression of Le(Y) in RMG-1-hFUT cells was about 20-fold that in RMG-1 cells, and that of integrin alpha5beta1 and an integrin-mediated signal transduction molecule, focal adhesion kinase, was also increased in RMG-1-hFUT cells. Interestingly, anti-Le(Y) antibodies were revealed to immunoprecipitate integrin alpha5beta1, indicating that its oligosaccharides are composed of Le(Y), the amounts of which was substantially elevated in RMG-1-hFUT cells. The adhesion and spreading potentials on fibronectin-coated plates of RMG-1-hFUT cells were significantly enhanced in comparison to those of RMG-1 cells, and were greatly suppressed by anti-Le(Y) antibodies, indicating that Le(Y) is involved in the integrin-fibronectin interaction. These results suggested that transfection of the alpha1,2-fucosyltransferase gene into ovarian carcinoma-derived cells brought about elevated expression of integrin alpha5beta1 with Le(Y), resulting in enhancement of the adhesion and spreading potentials of cells through the integrin-fibronection interaction, which was inhibited by anti-Le(Y) antibodies. Thus, Le(Y) in integrin alpha5beta1 was thought to be involved in the enhanced cell adhesion properties of malignant ovarian carcinomas."xsd:string |
| http://purl.uniprot.org/citations/20172014 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.biochi.2010.02.012"xsd:string |
| http://purl.uniprot.org/citations/20172014 | http://purl.uniprot.org/core/author | "Gao S."xsd:string |
| http://purl.uniprot.org/citations/20172014 | http://purl.uniprot.org/core/author | "Qi Y."xsd:string |
| http://purl.uniprot.org/citations/20172014 | http://purl.uniprot.org/core/author | "Lin B."xsd:string |
| http://purl.uniprot.org/citations/20172014 | http://purl.uniprot.org/core/author | "Liu S.C."xsd:string |
| http://purl.uniprot.org/citations/20172014 | http://purl.uniprot.org/core/author | "Zhang S.L."xsd:string |
| http://purl.uniprot.org/citations/20172014 | http://purl.uniprot.org/core/author | "Wang C.Z."xsd:string |
| http://purl.uniprot.org/citations/20172014 | http://purl.uniprot.org/core/author | "Iwamori M."xsd:string |
| http://purl.uniprot.org/citations/20172014 | http://purl.uniprot.org/core/author | "Zhu L.C."xsd:string |
| http://purl.uniprot.org/citations/20172014 | http://purl.uniprot.org/core/author | "Hao Y.Y."xsd:string |
| http://purl.uniprot.org/citations/20172014 | http://purl.uniprot.org/core/author | "Yan L.M."xsd:string |
| http://purl.uniprot.org/citations/20172014 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20172014 | http://purl.uniprot.org/core/name | "Biochimie"xsd:string |
| http://purl.uniprot.org/citations/20172014 | http://purl.uniprot.org/core/pages | "852-857"xsd:string |
| http://purl.uniprot.org/citations/20172014 | http://purl.uniprot.org/core/title | "Enhancement of the adhesive and spreading potentials of ovarian carcinoma RMG-1 cells due to increased expression of integrin alpha5beta1 with the Lewis Y-structure on transfection of the alpha1,2-fucosyltransferase gene."xsd:string |
| http://purl.uniprot.org/citations/20172014 | http://purl.uniprot.org/core/volume | "92"xsd:string |
| http://purl.uniprot.org/citations/20172014 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20172014 |
| http://purl.uniprot.org/citations/20172014 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20172014 |
| http://purl.uniprot.org/uniprot/#_A8K2L2-mappedCitation-20172014 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20172014 |
| http://purl.uniprot.org/uniprot/#_A0A7L9AYG2-mappedCitation-20172014 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20172014 |
| http://purl.uniprot.org/uniprot/#_A0A7L9AYP3-mappedCitation-20172014 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20172014 |
| http://purl.uniprot.org/uniprot/#_A0A7L9AZK5-mappedCitation-20172014 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20172014 |
| http://purl.uniprot.org/uniprot/#_A8K6A5-mappedCitation-20172014 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20172014 |