| http://purl.uniprot.org/citations/20177058 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20177058 | http://www.w3.org/2000/01/rdf-schema#comment | "In Drosophila, naked cuticle is an inducible antagonist of the Wnt-beta-catenin pathway, likely acting at the level of Dishevelled (Dsh/Dvl), an essential component of this pathway. The mechanism by which naked cuticle and its two vertebrate orthologs, Naked1 (NKD1) and Naked2 (NKD2), inhibit Dvl function is unknown. NKD2 is myristoylated, a co-translational modification that leads to its plasma membrane localization. In contrast, myristoylation-deficient G2A NKD2 is cytoplasmic. Herein we show that the ability of Nkd2/NKD2 to antagonize Wnt-beta-catenin activity during zebrafish embryonic development and in mammalian HEK293 cells is myristoylation-dependent. NKD2 and Dvl-1 interact and co-localize at the lateral membrane of polarized epithelial cells. In reciprocal overexpression and siRNA knockdown experiments, NKD2 and Dvl-1 destabilize each other via enhanced polyubiquitylation; this effect is also dependent upon Naked2 myristoylation. Cell fractionation and ubiquitylation assays indicate that endogenous NKD2 interacts with a slower migrating, ubiquitylated form of Dvl-1 in plasma membrane fractions. These results provide a mechanism by which NKD2 antagonizes Wnt signaling: myristoylated NKD2 interacts with Dvl-1 at the plasma membrane, and this interaction leads to their mutual ubiquitin-mediated proteasomal degradation."xsd:string |
| http://purl.uniprot.org/citations/20177058 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m109.075945"xsd:string |
| http://purl.uniprot.org/citations/20177058 | http://purl.uniprot.org/core/author | "Cao Z."xsd:string |
| http://purl.uniprot.org/citations/20177058 | http://purl.uniprot.org/core/author | "Li C."xsd:string |
| http://purl.uniprot.org/citations/20177058 | http://purl.uniprot.org/core/author | "Smith J.G."xsd:string |
| http://purl.uniprot.org/citations/20177058 | http://purl.uniprot.org/core/author | "Hu T."xsd:string |
| http://purl.uniprot.org/citations/20177058 | http://purl.uniprot.org/core/author | "Solnica-Krezel L."xsd:string |
| http://purl.uniprot.org/citations/20177058 | http://purl.uniprot.org/core/author | "Coffey R.J."xsd:string |
| http://purl.uniprot.org/citations/20177058 | http://purl.uniprot.org/core/author | "Van Raay T.J."xsd:string |
| http://purl.uniprot.org/citations/20177058 | http://purl.uniprot.org/core/author | "Willert K."xsd:string |
| http://purl.uniprot.org/citations/20177058 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20177058 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/20177058 | http://purl.uniprot.org/core/pages | "13561-13568"xsd:string |
| http://purl.uniprot.org/citations/20177058 | http://purl.uniprot.org/core/title | "Myristoylated Naked2 antagonizes Wnt-beta-catenin activity by degrading Dishevelled-1 at the plasma membrane."xsd:string |
| http://purl.uniprot.org/citations/20177058 | http://purl.uniprot.org/core/volume | "285"xsd:string |
| http://purl.uniprot.org/citations/20177058 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20177058 |
| http://purl.uniprot.org/citations/20177058 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20177058 |
| http://purl.uniprot.org/uniprot/O14640#attribution-30C9A78410C1AC56545DF3623C46618B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20177058 |
| http://purl.uniprot.org/uniprot/O14640#attribution-3F23E2BD2627121351F9063CFD0561B0 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20177058 |
| http://purl.uniprot.org/uniprot/A4ZNR4#attribution-4EBA74768CF26A1D036CE77A6AB6AD3C | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20177058 |
| http://purl.uniprot.org/uniprot/Q969F2#attribution-30C9A78410C1AC56545DF3623C46618B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20177058 |
| http://purl.uniprot.org/uniprot/Q969F2#attribution-3F23E2BD2627121351F9063CFD0561B0 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20177058 |
| http://purl.uniprot.org/uniprot/#_A4ZNR4-mappedCitation-20177058 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20177058 |
| http://purl.uniprot.org/uniprot/#_O14640-mappedCitation-20177058 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20177058 |