| http://purl.uniprot.org/citations/20203208 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20203208 | http://www.w3.org/2000/01/rdf-schema#comment | "Sialic acid-binding Ig superfamily lectins (Siglecs) are members of the Ig superfamily that recognize sialic acid residues of glycoproteins. Siglec-11 is a recently identified human-specific CD33-related Siglec that binds to alpha2,8-linked polysialic acids and is expressed on microglia, the brain resident innate immune cells. Polysialylated neuronal cell adhesion molecule (PSA-NCAM) is a putative ligand of Siglec-11. We observed gene transcription and protein expression of Siglec-11 splice variant 2 in human brain tissue samples by RT-PCR and Western blot analysis. Siglec-11 was detected on microglia in human brain tissue by immunohistochemistry. Human Siglec-11 splice variant 2 was ectopically expressed by a lentiviral vector system in cultured murine microglial cells. Stimulation of Siglec-11 by cross-linking suppressed the lipopolysaccharides (LPS)-induced gene transcription of the proinflammatory mediators interleukin-1beta and nitric oxide synthase-2 in microglia. Furthermore, phagocytosis of apoptotic neuronal material was reduced in Siglec-11 transduced microglia. Expression of PSA-NCAM was detected on microglia and neurons by immunohistochemistry and RT-PCR. Coculture of microglia transduced with Siglec-11 and neurons demonstrated neuroprotective function of Siglec-11. The neuroprotective effect of Siglec-11 was dependent on polysialic acid (PSA) residues on neurons, but independent on PSA on microglia. Thus, data demonstrate that human Siglec-11 ectopically expressed on murine microglia interacts with PSA on neurons, reduces LPS-induced gene transcription of proinflammatory mediators, impairs phagocytosis and alleviates microglial neurotoxicity."xsd:string |
| http://purl.uniprot.org/citations/20203208 | http://purl.org/dc/terms/identifier | "doi:10.1523/jneurosci.3940-09.2010"xsd:string |
| http://purl.uniprot.org/citations/20203208 | http://purl.uniprot.org/core/author | "Wang Y."xsd:string |
| http://purl.uniprot.org/citations/20203208 | http://purl.uniprot.org/core/author | "Neumann H."xsd:string |
| http://purl.uniprot.org/citations/20203208 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20203208 | http://purl.uniprot.org/core/name | "J Neurosci"xsd:string |
| http://purl.uniprot.org/citations/20203208 | http://purl.uniprot.org/core/pages | "3482-3488"xsd:string |
| http://purl.uniprot.org/citations/20203208 | http://purl.uniprot.org/core/title | "Alleviation of neurotoxicity by microglial human Siglec-11."xsd:string |
| http://purl.uniprot.org/citations/20203208 | http://purl.uniprot.org/core/volume | "30"xsd:string |
| http://purl.uniprot.org/citations/20203208 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20203208 |
| http://purl.uniprot.org/citations/20203208 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20203208 |
| http://purl.uniprot.org/uniprot/#_Q96RL6-mappedCitation-20203208 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20203208 |
| http://purl.uniprot.org/uniprot/Q96RL6 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20203208 |