| http://purl.uniprot.org/citations/20210363 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20210363 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20210363 | http://www.w3.org/2000/01/rdf-schema#comment | "ABCG5 and ABCG8 are half-size ABC transporters that function as heterodimers (ABCG5/G8) to reduce sterol absorption in the intestines and increase sterol excretion from the liver. Previous studies demonstrated that bile acids increased ABCG5/G8 specific cholesterol efflux in cell models. In this study we tested the effects of bile acids on ATP hydrolysis in Pichia pastoris purified ABCG5/G8 and found that they stimulated hydrolysis approximately 20-fold in wild-type ABCG5/G8 but not in a hydrolysis-deficient mutant. Nonconjugated cholate supported the highest ATPase activity in ABCG5/G8 (256 +/-9 nmol min(-1) mg(-1)). ATP hydrolysis was also stimulated by other conjugated bile acids and a mixture of bile acids resembling human bile with activities ranging from 129 +/- 4 to 147 +/-14 nmol min(-1) mg(-1). The kinetic parameters, inhibitor profiles, and lipid requirements of bile acid stimulated ATP hydrolysis were characterized. Cholate-stimulated ATP hydrolysis was maximal at concentrations of >or=10 mM MgATP and had a relatively high K(M) (MgATP) of approximately 1 mM. Orthovanadate, BeFx, and AlFx effectively inhibited ABCG5/G8 at concentrations of 1 mM. Various lipid mixtures supported bile acid-stimulated ATP hydrolysis, which increased when cholesterol was present. The data demonstrate that bile acids together with lipids and cholesterol increase ATP hydrolysis in purified ABCG5/G8. Bile acids may promote an active conformation of purified ABCG5/G8 either by global stabilization of the transporter or by binding to a specific site on ABCG5/G8."xsd:string |
| http://purl.uniprot.org/citations/20210363 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi902064g"xsd:string |
| http://purl.uniprot.org/citations/20210363 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi902064g"xsd:string |
| http://purl.uniprot.org/citations/20210363 | http://purl.uniprot.org/core/author | "Lee J.Y."xsd:string |
| http://purl.uniprot.org/citations/20210363 | http://purl.uniprot.org/core/author | "Lee J.Y."xsd:string |
| http://purl.uniprot.org/citations/20210363 | http://purl.uniprot.org/core/author | "Johnson B.J."xsd:string |
| http://purl.uniprot.org/citations/20210363 | http://purl.uniprot.org/core/author | "Johnson B.J."xsd:string |
| http://purl.uniprot.org/citations/20210363 | http://purl.uniprot.org/core/author | "Pickert A."xsd:string |
| http://purl.uniprot.org/citations/20210363 | http://purl.uniprot.org/core/author | "Pickert A."xsd:string |
| http://purl.uniprot.org/citations/20210363 | http://purl.uniprot.org/core/author | "Urbatsch I.L."xsd:string |
| http://purl.uniprot.org/citations/20210363 | http://purl.uniprot.org/core/author | "Urbatsch I.L."xsd:string |
| http://purl.uniprot.org/citations/20210363 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20210363 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20210363 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/20210363 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/20210363 | http://purl.uniprot.org/core/pages | "3403-3411"xsd:string |
| http://purl.uniprot.org/citations/20210363 | http://purl.uniprot.org/core/pages | "3403-3411"xsd:string |
| http://purl.uniprot.org/citations/20210363 | http://purl.uniprot.org/core/title | "Bile acids stimulate ATP hydrolysis in the purified cholesterol transporter ABCG5/G8."xsd:string |
| http://purl.uniprot.org/citations/20210363 | http://purl.uniprot.org/core/title | "Bile acids stimulate ATP hydrolysis in the purified cholesterol transporter ABCG5/G8."xsd:string |
| http://purl.uniprot.org/citations/20210363 | http://purl.uniprot.org/core/volume | "49"xsd:string |
| http://purl.uniprot.org/citations/20210363 | http://purl.uniprot.org/core/volume | "49"xsd:string |
| http://purl.uniprot.org/citations/20210363 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20210363 |
| http://purl.uniprot.org/citations/20210363 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20210363 |