| http://purl.uniprot.org/citations/20213681 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20213681 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20213681 | http://www.w3.org/2000/01/rdf-schema#comment | "To establish a strategy for the comprehensive identification of human N-myristoylated proteins, the susceptibility of human cDNA clones to protein N-myristoylation was evaluated by metabolic labeling and MS analyses of proteins expressed in an insect cell-free protein synthesis system. One-hundred-and-forty-one cDNA clones with N-terminal Met-Gly motifs were selected as potential candidates from approximately 2000 Kazusa ORFeome project human cDNA clones, and their susceptibility to protein N-myristoylation was evaluated using fusion proteins, in which the N-terminal ten amino acid residues were fused to an epitope-tagged model protein. As a result, the products of 29 out of 141 cDNA clones were found to be effectively N-myristoylated. The metabolic labeling experiments both in an insect cell-free protein synthesis system and in the transfected COS-1 cells using full-length cDNA revealed that 27 out of 29 proteins were in fact N-myristoylated. Database searches with these 27 cDNA clones revealed that 18 out of 27 proteins are novel N-myristoylated proteins that have not been reported previously to be N-myristoylated, indicating that this strategy is useful for the comprehensive identification of human N-myristoylated proteins from human cDNA resources."xsd:string |
| http://purl.uniprot.org/citations/20213681 | http://purl.org/dc/terms/identifier | "doi:10.1002/pmic.200900783"xsd:string |
| http://purl.uniprot.org/citations/20213681 | http://purl.org/dc/terms/identifier | "doi:10.1002/pmic.200900783"xsd:string |
| http://purl.uniprot.org/citations/20213681 | http://purl.uniprot.org/core/author | "Suzuki T."xsd:string |
| http://purl.uniprot.org/citations/20213681 | http://purl.uniprot.org/core/author | "Suzuki T."xsd:string |
| http://purl.uniprot.org/citations/20213681 | http://purl.uniprot.org/core/author | "Tsunasawa S."xsd:string |
| http://purl.uniprot.org/citations/20213681 | http://purl.uniprot.org/core/author | "Tsunasawa S."xsd:string |
| http://purl.uniprot.org/citations/20213681 | http://purl.uniprot.org/core/author | "Moriya K."xsd:string |
| http://purl.uniprot.org/citations/20213681 | http://purl.uniprot.org/core/author | "Moriya K."xsd:string |
| http://purl.uniprot.org/citations/20213681 | http://purl.uniprot.org/core/author | "Utsumi T."xsd:string |
| http://purl.uniprot.org/citations/20213681 | http://purl.uniprot.org/core/author | "Utsumi T."xsd:string |
| http://purl.uniprot.org/citations/20213681 | http://purl.uniprot.org/core/author | "Ota Y."xsd:string |
| http://purl.uniprot.org/citations/20213681 | http://purl.uniprot.org/core/author | "Ota Y."xsd:string |
| http://purl.uniprot.org/citations/20213681 | http://purl.uniprot.org/core/author | "Ando E."xsd:string |
| http://purl.uniprot.org/citations/20213681 | http://purl.uniprot.org/core/author | "Ando E."xsd:string |
| http://purl.uniprot.org/citations/20213681 | http://purl.uniprot.org/core/author | "Ezure T."xsd:string |
| http://purl.uniprot.org/citations/20213681 | http://purl.uniprot.org/core/author | "Ezure T."xsd:string |
| http://purl.uniprot.org/citations/20213681 | http://purl.uniprot.org/core/author | "Nagatoshi K."xsd:string |
| http://purl.uniprot.org/citations/20213681 | http://purl.uniprot.org/core/author | "Nagatoshi K."xsd:string |
| http://purl.uniprot.org/citations/20213681 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20213681 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20213681 | http://purl.uniprot.org/core/name | "Proteomics"xsd:string |
| http://purl.uniprot.org/citations/20213681 | http://purl.uniprot.org/core/name | "Proteomics"xsd:string |