| http://purl.uniprot.org/citations/20220555 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20220555 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundHuman adipose-derived stromal cells possess a great potential for tissue engineering purposes. The authors' laboratory is interested in harnessing human adipose-derived stromal cells for skeletal tissue regeneration and identifying those factors that enhance human adipose-derived stromal cell osteogenic differentiation. The authors hypothesized that insulin-like growth factor (IGF) and platelet-derived growth factor (PDGF) would stimulate human adipose-derived stromal cell osteogenesis and that IGF would stimulate adipogenesis.MethodsAdipose-derived stromal cells were harvested from human lipoaspirate. Previously, a microarray analysis examined gene expression throughout osteogenic differentiation. In a candidate fashion, the authors added recombinant IGF-1 and PDGF-alpha individually and in combination. Osteogenesis and adipogenesis were assessed by alkaline phosphatase, alizarin red, and oil red O staining, and quantitative real-time polymerase chain reaction (RUNX2, ALP, OCN, IGF1, PPARG, LPL, AP2, and GCP1). Finally, intersection between IGF and PDGF signaling pathways was evaluated.ResultsIGF-1 was observed to increase osteogenic differentiation by all markers (p < 0.01). However, PDGF-alpha when added alone primarily did not affect osteogenic markers. PDGF-alpha positively regulated transcription of IGF1. Addition of PDGF-alpha in combination with or before IGF-1 enhanced osteogenesis more than either alone. IGF-1 increased whereas PDGF-alpha diminished human adipose-derived stromal cell adipogenesis.ConclusionsIGF signaling significantly increased osteogenesis in human adipose-derived stromal cells and may be used for tissue-engineering purposes. The combination of PDGF and IGF may be more beneficial than either alone in driving adipose-derived stromal cell osteogenesis. Future in vivo applications will focus on the combination of adipose-derived stromal cells, biomimetic scaffolds, and recombinant IGF."xsd:string |
| http://purl.uniprot.org/citations/20220555 | http://purl.org/dc/terms/identifier | "doi:10.1097/prs.0b013e3181da8858"xsd:string |
| http://purl.uniprot.org/citations/20220555 | http://purl.uniprot.org/core/author | "Longaker M.T."xsd:string |
| http://purl.uniprot.org/citations/20220555 | http://purl.uniprot.org/core/author | "Levi B."xsd:string |
| http://purl.uniprot.org/citations/20220555 | http://purl.uniprot.org/core/author | "Wan D.C."xsd:string |
| http://purl.uniprot.org/citations/20220555 | http://purl.uniprot.org/core/author | "James A.W."xsd:string |
| http://purl.uniprot.org/citations/20220555 | http://purl.uniprot.org/core/author | "Commons G.W."xsd:string |
| http://purl.uniprot.org/citations/20220555 | http://purl.uniprot.org/core/author | "Glotzbach J.P."xsd:string |
| http://purl.uniprot.org/citations/20220555 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20220555 | http://purl.uniprot.org/core/name | "Plast Reconstr Surg"xsd:string |
| http://purl.uniprot.org/citations/20220555 | http://purl.uniprot.org/core/pages | "41-52"xsd:string |
| http://purl.uniprot.org/citations/20220555 | http://purl.uniprot.org/core/title | "Regulation of human adipose-derived stromal cell osteogenic differentiation by insulin-like growth factor-1 and platelet-derived growth factor-alpha."xsd:string |
| http://purl.uniprot.org/citations/20220555 | http://purl.uniprot.org/core/volume | "126"xsd:string |
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