| http://purl.uniprot.org/citations/20224290 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20224290 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20224290 | http://www.w3.org/2000/01/rdf-schema#comment | "In ventricular myocytes, α1-AR stimulates Gαs proteins and reduces the transient outward K(+) current (I(to)) via a cAMP/PKA-mediated pathway and thus regulates cardiac contraction and excitability. This I(to) reduction is compartmentalized and limited to discrete membrane regions since PKA-dependent phosphorylation of the I(to) channels after α1-AR stimulation requires the integrity of both the sarcoplasmic membrane and the cytoskeleton. The aim of this work was to investigate the mechanisms involved in the compartmentalization of the PKA-dependent modulation of I(to) in response to α1-AR activation. I(to) current recordings were performed by the Patch-Clamp technique. Membrane rafts from isolated ventricular myocytes were extracted by centrifugation in a sucrose density gradient. The different proteins were visualized by western blot and protein-protein interactions determined by coimmunoprecipitation experiments. Localization of I(to) channel in caveolae, particular subtypes of membrane rafts, was achieved by electron microscopy. Patch-Clamp recordings show that a functional supramolecular complex, kept together by the Akinase anchoring protein AKAP100, exist in caveolae in living myocytes. Density gradients and immunoprecipitation experiments show that the components of the α1-AR/I(to) pathway localize in caveolae, forming two different groups of proteins. The K(V)4.2/K(V)4.3 channel forms a supramolecular complex with PKA through AKAP100 and is attached to caveolae by interacting with caveolin-3. On the other hand, α1-AR, Gαs and adenylate cyclase gather in a second group also connected to caveolin-3. Therefore, both groups of preassembled proteins are maintained in close proximity by caveolin-3. A different I(to) channel population localizes in non-caveolar membrane rafts and is not sensitive to α1-adrenergic regulation."xsd:string |
| http://purl.uniprot.org/citations/20224290 | http://purl.org/dc/terms/identifier | "doi:10.4161/chan.4.3.11479"xsd:string |
| http://purl.uniprot.org/citations/20224290 | http://purl.org/dc/terms/identifier | "doi:10.4161/chan.4.3.11479"xsd:string |
| http://purl.uniprot.org/citations/20224290 | http://purl.uniprot.org/core/author | "Casis O."xsd:string |
| http://purl.uniprot.org/citations/20224290 | http://purl.uniprot.org/core/author | "Casis O."xsd:string |
| http://purl.uniprot.org/citations/20224290 | http://purl.uniprot.org/core/author | "Gallego M."xsd:string |
| http://purl.uniprot.org/citations/20224290 | http://purl.uniprot.org/core/author | "Gallego M."xsd:string |
| http://purl.uniprot.org/citations/20224290 | http://purl.uniprot.org/core/author | "Alday A."xsd:string |
| http://purl.uniprot.org/citations/20224290 | http://purl.uniprot.org/core/author | "Alday A."xsd:string |
| http://purl.uniprot.org/citations/20224290 | http://purl.uniprot.org/core/author | "Urrutia J."xsd:string |
| http://purl.uniprot.org/citations/20224290 | http://purl.uniprot.org/core/author | "Urrutia J."xsd:string |
| http://purl.uniprot.org/citations/20224290 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20224290 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20224290 | http://purl.uniprot.org/core/name | "Channels"xsd:string |
| http://purl.uniprot.org/citations/20224290 | http://purl.uniprot.org/core/name | "Channels"xsd:string |
| http://purl.uniprot.org/citations/20224290 | http://purl.uniprot.org/core/pages | "168-178"xsd:string |
| http://purl.uniprot.org/citations/20224290 | http://purl.uniprot.org/core/pages | "168-178"xsd:string |
| http://purl.uniprot.org/citations/20224290 | http://purl.uniprot.org/core/title | "Alpha1-adrenoceptors regulate only the caveolae-located subpopulation of cardiac K(V)4 channels."xsd:string |
| http://purl.uniprot.org/citations/20224290 | http://purl.uniprot.org/core/title | "Alpha1-adrenoceptors regulate only the caveolae-located subpopulation of cardiac K(V)4 channels."xsd:string |
| http://purl.uniprot.org/citations/20224290 | http://purl.uniprot.org/core/volume | "4"xsd:string |
| http://purl.uniprot.org/citations/20224290 | http://purl.uniprot.org/core/volume | "4"xsd:string |
| http://purl.uniprot.org/citations/20224290 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20224290 |
| http://purl.uniprot.org/citations/20224290 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20224290 |