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http://purl.uniprot.org/citations/20225396http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20225396http://www.w3.org/2000/01/rdf-schema#comment"

Aims/hypothesis

Glucagon-like peptide-1 receptor (GLP-1R) agonists improve glucose control in animals and humans with type 1 diabetes. However, there is little information on the role of the GLP-1R in the immune system. We studied the role of the GLP-1R in immune function in wild-type (WT) and nonobese diabetic (NOD) and Glp1r-/-mice.

Methods

Glp1r mRNA expression was examined in sorted immune subpopulations by RT-PCR. The effects of GLP-1R activation were assessed on cAMP production and proliferation, migration and survival of primary immune cells from WT and NOD mice. The ability of primary cells from Glp1r-/-mice to proliferate, migrate or survive apoptosis was determined. Immunophenotyping studies were performed to assess the frequency of immune subpopulations in Glp1r-/-mice.

Results

Ex vivo activation of the GLP-1R resulted in a modest but significant elevation of cAMP in primary thymocytes and splenocytes from both WT and NOD mice. GLP-1R activation did not increase proliferation of primary thymocytes, splenocytes or peripheral lymph node cells. In contrast, Glp1r-/-thymocytes exhibited a hypoproliferative response, whilst peripheral Glp1r-/-lymphocytes were hyperproliferative in response to mitogenic stimulation. Activation or loss of GLP-1R signalling did not modify apoptosis or chemotaxis in primary lymphocytes. Male Glp1r-/-mice exhibited a significantly lower percentage of peripheral regulatory T cells, although no differences were observed in the numbers of CD4+ and CD8+ T cells and B cells in the spleen and lymph nodes of Glp1r-/-mice.

Conclusions/interpretation

These studies establish that GLP-1R signalling may regulate lymphocyte proliferation and maintenance of peripheral regulatory T cells."xsd:string
http://purl.uniprot.org/citations/20225396http://purl.org/dc/terms/identifier"doi:10.1007/s00125-009-1643-x"xsd:string
http://purl.uniprot.org/citations/20225396http://purl.uniprot.org/core/author"Siminovitch K.A."xsd:string
http://purl.uniprot.org/citations/20225396http://purl.uniprot.org/core/author"Drucker D.J."xsd:string
http://purl.uniprot.org/citations/20225396http://purl.uniprot.org/core/author"Danska J.S."xsd:string
http://purl.uniprot.org/citations/20225396http://purl.uniprot.org/core/author"Hadjiyanni I."xsd:string
http://purl.uniprot.org/citations/20225396http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/20225396http://purl.uniprot.org/core/name"Diabetologia"xsd:string
http://purl.uniprot.org/citations/20225396http://purl.uniprot.org/core/pages"730-740"xsd:string
http://purl.uniprot.org/citations/20225396http://purl.uniprot.org/core/title"Glucagon-like peptide-1 receptor signalling selectively regulates murine lymphocyte proliferation and maintenance of peripheral regulatory T cells."xsd:string
http://purl.uniprot.org/citations/20225396http://purl.uniprot.org/core/volume"53"xsd:string
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