| http://purl.uniprot.org/citations/20228168 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20228168 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20228168 | http://www.w3.org/2000/01/rdf-schema#comment | "Previously, we showed that levels of different CCAAT/enhancer binding protein (C/EBP) mRNAs in the liver of rainbow trout were modulated by GH and suggested that C/EBPs might be involved in GH-induced IGF-II gene expression. As a step toward further investigation, we have developed monospecific polyclonal antibodies to detect rainbow trout C/EBPalpha, -beta1, -beta2, and -delta2 isoform proteins. Injection of GH into adult rainbow trout resulted in a significant increase of C/EBPbeta1, C/EBPbeta2, and C/EBPdelta2 proteins in the liver. Chromatin immunoprecipitation analysis revealed that C/EBPbeta2 binds to multiple sites at the 5' promoter/regulatory region, introns, and the 3' untranslated region of the IGF-II gene. GH treatment reduced C/EBPbeta2 binding to several of these regions at 6 h after injection. The decreased occupancy of C/EBPbeta2 coincided well with an increase of histone H4 acetylation at the proximal promoter and elevation of the IGF-II mRNA level. Immunoblotting analysis showed that C/EBPbeta2 existed predominately as a truncated form in the liver, and cotransfection analysis further showed that the truncated C/EBPbeta2 acted as a negative regulator on IGF-II proximal promoter. GH treatment caused deacetylation of C/EBPbeta2 in the liver. In addition, we observed a GH-dependent interaction of C/EBPbeta2 with a complex involving histone H1. All together, these results suggest that C/EBPbeta2 was regulated at multiple levels by GH, and C/EBPbeta2 may play a suppressive role in mediating GH-induced IGF-II expression in the liver of rainbow trout."xsd:string |
| http://purl.uniprot.org/citations/20228168 | http://purl.org/dc/terms/identifier | "doi:10.1210/en.2009-0960"xsd:string |
| http://purl.uniprot.org/citations/20228168 | http://purl.org/dc/terms/identifier | "doi:10.1210/en.2009-0960"xsd:string |
| http://purl.uniprot.org/citations/20228168 | http://purl.uniprot.org/core/author | "Chen T.T."xsd:string |
| http://purl.uniprot.org/citations/20228168 | http://purl.uniprot.org/core/author | "Chen T.T."xsd:string |
| http://purl.uniprot.org/citations/20228168 | http://purl.uniprot.org/core/author | "Lo J.H."xsd:string |
| http://purl.uniprot.org/citations/20228168 | http://purl.uniprot.org/core/author | "Lo J.H."xsd:string |
| http://purl.uniprot.org/citations/20228168 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20228168 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20228168 | http://purl.uniprot.org/core/name | "Endocrinology"xsd:string |
| http://purl.uniprot.org/citations/20228168 | http://purl.uniprot.org/core/name | "Endocrinology"xsd:string |
| http://purl.uniprot.org/citations/20228168 | http://purl.uniprot.org/core/pages | "2128-2139"xsd:string |
| http://purl.uniprot.org/citations/20228168 | http://purl.uniprot.org/core/pages | "2128-2139"xsd:string |
| http://purl.uniprot.org/citations/20228168 | http://purl.uniprot.org/core/title | "CCAAT/enhancer binding protein beta2 is involved in growth hormone-regulated insulin-like growth factor-II gene expression in the liver of rainbow trout (Oncorhynchus mykiss)."xsd:string |
| http://purl.uniprot.org/citations/20228168 | http://purl.uniprot.org/core/title | "CCAAT/enhancer binding protein beta2 is involved in growth hormone-regulated insulin-like growth factor-II gene expression in the liver of rainbow trout (Oncorhynchus mykiss)."xsd:string |
| http://purl.uniprot.org/citations/20228168 | http://purl.uniprot.org/core/volume | "151"xsd:string |
| http://purl.uniprot.org/citations/20228168 | http://purl.uniprot.org/core/volume | "151"xsd:string |
| http://purl.uniprot.org/citations/20228168 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20228168 |
| http://purl.uniprot.org/citations/20228168 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20228168 |
| http://purl.uniprot.org/citations/20228168 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20228168 |
| http://purl.uniprot.org/citations/20228168 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20228168 |
| http://purl.uniprot.org/uniprot/C6GBY8 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/20228168 |
| http://purl.uniprot.org/uniprot/#_kb.C6GBY8_up.isolatedFrom_tissue.642 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/20228168 |