| http://purl.uniprot.org/citations/20231318 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20231318 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20231318 | http://www.w3.org/2000/01/rdf-schema#comment | "Human chromosome end-capping and telomerase regulation require POT1 (Protection of Telomeres 1) and TPP1 proteins, which bind to the 3' ssDNA extension of human telomeres. POT1-TPP1 binding to telomeric DNA activates telomerase repeat addition processivity. We now provide evidence that this POT1-TPP1 activation requires specific interactions with telomerase, rather than it being a DNA substrate-specific effect. First, telomerase from the fish medaka, which extends the same telomeric DNA primer as human telomerase, was not activated by human POT1-TPP1. Second, mutation of a conserved glycine, Gly100 in the TEN (telomerase essential N-terminal) domain of TERT, abolished the enhancement of telomerase processivity by POT1-TPP1, in contrast to other single amino acid mutations. Chimeric human-fish telomerases that contained the human TEN domain were active but not stimulated by POT1-TPP1, showing that additional determinants of processivity lie outside the TEN domain. Finally, primers bound to mouse POT1A and human TPP1 were activated for extension by human telomerase, whereas mPOT1A-mTPP1 was most active with mouse telomerase, indicating that these mammalian telomerases have specificity for their respective TPP1 proteins. We suggest that a sequence-specific interaction between TPP1 in the TPP1-POT1-telomeric DNA complex and the G100 region of the TEN domain of TERT is necessary for high-processivity telomerase action."xsd:string |
| http://purl.uniprot.org/citations/20231318 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.1881810"xsd:string |
| http://purl.uniprot.org/citations/20231318 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.1881810"xsd:string |
| http://purl.uniprot.org/citations/20231318 | http://purl.uniprot.org/core/author | "Cech T.R."xsd:string |
| http://purl.uniprot.org/citations/20231318 | http://purl.uniprot.org/core/author | "Cech T.R."xsd:string |
| http://purl.uniprot.org/citations/20231318 | http://purl.uniprot.org/core/author | "Nandakumar J."xsd:string |
| http://purl.uniprot.org/citations/20231318 | http://purl.uniprot.org/core/author | "Nandakumar J."xsd:string |
| http://purl.uniprot.org/citations/20231318 | http://purl.uniprot.org/core/author | "Podell E.R."xsd:string |
| http://purl.uniprot.org/citations/20231318 | http://purl.uniprot.org/core/author | "Podell E.R."xsd:string |
| http://purl.uniprot.org/citations/20231318 | http://purl.uniprot.org/core/author | "Zaug A.J."xsd:string |
| http://purl.uniprot.org/citations/20231318 | http://purl.uniprot.org/core/author | "Zaug A.J."xsd:string |
| http://purl.uniprot.org/citations/20231318 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20231318 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20231318 | http://purl.uniprot.org/core/name | "Genes Dev."xsd:string |
| http://purl.uniprot.org/citations/20231318 | http://purl.uniprot.org/core/name | "Genes Dev."xsd:string |
| http://purl.uniprot.org/citations/20231318 | http://purl.uniprot.org/core/pages | "613-622"xsd:string |
| http://purl.uniprot.org/citations/20231318 | http://purl.uniprot.org/core/pages | "613-622"xsd:string |
| http://purl.uniprot.org/citations/20231318 | http://purl.uniprot.org/core/title | "Functional interaction between telomere protein TPP1 and telomerase."xsd:string |
| http://purl.uniprot.org/citations/20231318 | http://purl.uniprot.org/core/title | "Functional interaction between telomere protein TPP1 and telomerase."xsd:string |
| http://purl.uniprot.org/citations/20231318 | http://purl.uniprot.org/core/volume | "24"xsd:string |
| http://purl.uniprot.org/citations/20231318 | http://purl.uniprot.org/core/volume | "24"xsd:string |
| http://purl.uniprot.org/citations/20231318 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20231318 |
| http://purl.uniprot.org/citations/20231318 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20231318 |