| http://purl.uniprot.org/citations/20238167 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20238167 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20238167 | http://www.w3.org/2000/01/rdf-schema#comment | "Salsola kali pollen is a common cause of pollinosis during summer and early fall in desert and semi-desert regions. The aim of this study was the identification and characterization of Sal k 3, a new allergen from S. kali pollen. S. kali pollen extract was fractionated by SDS-PAGE and the allergenic profile was determined by IgE-immunoblotting using twelve S. kali allergic patients. Protein identification was carried out by the means of mass spectrometry. Using degenerated primers, two DNA fragments encoding N- and C-terminal domain of Sal k 3 were amplified by PCR, then cloned into the PTZ57R/T vector and sequenced. The open reading frame of Sal k 3 fragments were subcloned in the pET-32b(+) vector, expressed in E. coli, and purified by Ni2+ affinity chromatography. The IgE-binding capacity of rSal k 3 fragments was then studied by IgE-immunoblotting, inhibition assays, and skin prick tests. A 45-kDa allergen was identified as a fragment of the cobalamin-independent methionine synthase (MetE) by mass spectrometry and was detected in the sera of 8/12 (66.6%) of S. kali allergic patients. Moreover, inhibition assays demonstrated that the purified rSal k 3 fragments were similar to their counterparts in the crude extract. Sal k 3 represents a new allergen of S. kali pollen and seems to be an important allergenic compound in S. kali pollen."xsd:string |
| http://purl.uniprot.org/citations/20238167 | http://purl.org/dc/terms/identifier | "doi:10.1007/s11033-010-0078-2"xsd:string |
| http://purl.uniprot.org/citations/20238167 | http://purl.org/dc/terms/identifier | "doi:10.1007/s11033-010-0078-2"xsd:string |
| http://purl.uniprot.org/citations/20238167 | http://purl.uniprot.org/core/author | "Sankian M."xsd:string |
| http://purl.uniprot.org/citations/20238167 | http://purl.uniprot.org/core/author | "Sankian M."xsd:string |
| http://purl.uniprot.org/citations/20238167 | http://purl.uniprot.org/core/author | "Assarehzadegan M.A."xsd:string |
| http://purl.uniprot.org/citations/20238167 | http://purl.uniprot.org/core/author | "Assarehzadegan M.A."xsd:string |
| http://purl.uniprot.org/citations/20238167 | http://purl.uniprot.org/core/author | "Falak R."xsd:string |
| http://purl.uniprot.org/citations/20238167 | http://purl.uniprot.org/core/author | "Falak R."xsd:string |
| http://purl.uniprot.org/citations/20238167 | http://purl.uniprot.org/core/author | "Jabbari F."xsd:string |
| http://purl.uniprot.org/citations/20238167 | http://purl.uniprot.org/core/author | "Jabbari F."xsd:string |
| http://purl.uniprot.org/citations/20238167 | http://purl.uniprot.org/core/author | "Tehrani M."xsd:string |
| http://purl.uniprot.org/citations/20238167 | http://purl.uniprot.org/core/author | "Tehrani M."xsd:string |
| http://purl.uniprot.org/citations/20238167 | http://purl.uniprot.org/core/author | "Varasteh A."xsd:string |
| http://purl.uniprot.org/citations/20238167 | http://purl.uniprot.org/core/author | "Varasteh A."xsd:string |
| http://purl.uniprot.org/citations/20238167 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/20238167 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/20238167 | http://purl.uniprot.org/core/name | "Mol. Biol. Rep."xsd:string |
| http://purl.uniprot.org/citations/20238167 | http://purl.uniprot.org/core/name | "Mol. Biol. Rep."xsd:string |
| http://purl.uniprot.org/citations/20238167 | http://purl.uniprot.org/core/pages | "65-73"xsd:string |
| http://purl.uniprot.org/citations/20238167 | http://purl.uniprot.org/core/pages | "65-73"xsd:string |
| http://purl.uniprot.org/citations/20238167 | http://purl.uniprot.org/core/title | "Identification of methionine synthase (Sal k 3), as a novel allergen of Salsola kali pollen."xsd:string |
| http://purl.uniprot.org/citations/20238167 | http://purl.uniprot.org/core/title | "Identification of methionine synthase (Sal k 3), as a novel allergen of Salsola kali pollen."xsd:string |