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Subject | Predicate | Object |
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http://purl.uniprot.org/citations/2034675 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/2034675 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/2034675 | http://www.w3.org/2000/01/rdf-schema#comment | "Two types of cDNA for nonspecific lipid transfer protein (nsLTP), identical to sterol carrier protein 2, of rat liver were cloned; one was 787 base pairs (bp) long containing a 429-bp open reading frame of 143 amino acids, with a mass of 15,303 Da (15-kDa protein). The cDNA from the other type was 1966 bp long, including a 1641-bp open reading frame of 547 amino acids, giving a mass of 59,002 Da (60-kDa protein). The deduced primary sequence for the 15-kDa protein was exactly the same as the published sequence of purified nsLTP, except for an extra N-terminal sequence of 20 amino acids, consistent with the finding that nsLTP is synthesized as a larger precursor and processed to a mature form. The sequence for the 60-kDa protein contained, at the 3' end, the full sequence of the 15-kDa protein, a larger precursor to nsLTP. The 15- and 60-kDa proteins, synthesized in vitro from the respective cDNAs, were both immunoprecipitated by rabbit anti-rat liver nsLTP antibody and comigrated in SDS/PAGE with the proteins made in vitro from total liver RNA. These results shed new light on the dispute among several groups of investigators about the crossreactivity of anti-nsLTP antibody with a higher molecular mass, 60-kDa protein. In Northern blot analysis, two major RNA bands, 0.85 and 2.2 kilobases (kb) long, were detected together with two minor bands of 1.6 and 2.9 kb. The 0.85- and 2.2-kb RNAs most likely encode the 15-and 60-kDa proteins, respectively."xsd:string |
http://purl.uniprot.org/citations/2034675 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.88.10.4338"xsd:string |
http://purl.uniprot.org/citations/2034675 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.88.10.4338"xsd:string |
http://purl.uniprot.org/citations/2034675 | http://purl.uniprot.org/core/author | "Mori T."xsd:string |
http://purl.uniprot.org/citations/2034675 | http://purl.uniprot.org/core/author | "Mori T."xsd:string |
http://purl.uniprot.org/citations/2034675 | http://purl.uniprot.org/core/author | "Mori H."xsd:string |
http://purl.uniprot.org/citations/2034675 | http://purl.uniprot.org/core/author | "Mori H."xsd:string |
http://purl.uniprot.org/citations/2034675 | http://purl.uniprot.org/core/author | "Tashiro Y."xsd:string |
http://purl.uniprot.org/citations/2034675 | http://purl.uniprot.org/core/author | "Tashiro Y."xsd:string |
http://purl.uniprot.org/citations/2034675 | http://purl.uniprot.org/core/author | "Fujiki Y."xsd:string |
http://purl.uniprot.org/citations/2034675 | http://purl.uniprot.org/core/author | "Fujiki Y."xsd:string |
http://purl.uniprot.org/citations/2034675 | http://purl.uniprot.org/core/author | "Tsukamoto T."xsd:string |
http://purl.uniprot.org/citations/2034675 | http://purl.uniprot.org/core/author | "Tsukamoto T."xsd:string |
http://purl.uniprot.org/citations/2034675 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
http://purl.uniprot.org/citations/2034675 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
http://purl.uniprot.org/citations/2034675 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
http://purl.uniprot.org/citations/2034675 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
http://purl.uniprot.org/citations/2034675 | http://purl.uniprot.org/core/pages | "4338-4342"xsd:string |
http://purl.uniprot.org/citations/2034675 | http://purl.uniprot.org/core/pages | "4338-4342"xsd:string |
http://purl.uniprot.org/citations/2034675 | http://purl.uniprot.org/core/title | "Molecular cloning and deduced amino acid sequence of nonspecific lipid transfer protein (sterol carrier protein 2) of rat liver: a higher molecular mass (60 kDa) protein contains the primary sequence of nonspecific lipid transfer protein as its C-terminal part."xsd:string |
http://purl.uniprot.org/citations/2034675 | http://purl.uniprot.org/core/title | "Molecular cloning and deduced amino acid sequence of nonspecific lipid transfer protein (sterol carrier protein 2) of rat liver: a higher molecular mass (60 kDa) protein contains the primary sequence of nonspecific lipid transfer protein as its C-terminal part."xsd:string |
http://purl.uniprot.org/citations/2034675 | http://purl.uniprot.org/core/volume | "88"xsd:string |
http://purl.uniprot.org/citations/2034675 | http://purl.uniprot.org/core/volume | "88"xsd:string |