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http://purl.uniprot.org/citations/20346955http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20346955http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20346955http://www.w3.org/2000/01/rdf-schema#comment"TTN-1, a titin like protein in Caenorhabditis elegans, is encoded by a single gene and consists of multiple Ig and fibronectin 3 domains, a protein kinase domain and several regions containing tandem short repeat sequences. We have characterized TTN-1's sarcomere distribution, protein interaction with key myofibrillar proteins as well as the conformation malleability of representative motifs of five classes of short repeats. We report that two antibodies developed to portions of TTN-1 detect an approximately 2-MDa polypeptide on Western blots. In addition, by immunofluorescence staining, both of these antibodies localize to the I-band and may extend into the outer edge of the A-band in the obliquely striated muscle of the nematode. Six different 300-residue segments of TTN-1 were shown to variously interact with actin and/or myosin in vitro. Conformations of synthetic peptides of representative copies of each of the five classes of repeats--39-mer PEVT, 51-mer CEEEI, 42-mer AAPLE, 32-mer BLUE and 30-mer DispRep--were investigated by circular dichroism at different temperatures, ionic strengths and solvent polarities. The PEVT, CEEEI, DispRep and AAPLE peptides display a combination of a polyproline II helix and an unordered structure in aqueous solution and convert in trifluoroethanol to alpha-helix (PEVT, CEEEI, DispRep) and beta-turn (AAPLE) structures, respectively. The octads in BLUE motifs form unstable alpha-helix-like structures coils in aqueous solution and negligible heptad-based, alpha-helical coiled-coils. The alpha-helical structure, as modeled by threading and molecular dynamics simulations, tends to form helical bundles and crosses based on its 8-4-2-2 hydrophobic helical patterns and charge arrays on its surface. Our finding indicates that APPLE, PEVT, CEEEI and DispRep regions are all intrinsically disordered and highly reminiscent of the conformational malleability and elasticity of vertebrate titin PEVK segments. The proposed presence of long, modular and unstable alpha-helical oligomerization domains in the BLUE region of TTN-1 could bundle TTN-1 and stabilize oblique striation of the sarcomere."xsd:string
http://purl.uniprot.org/citations/20346955http://purl.org/dc/terms/identifier"doi:10.1016/j.jmb.2010.03.032"xsd:string
http://purl.uniprot.org/citations/20346955http://purl.org/dc/terms/identifier"doi:10.1016/j.jmb.2010.03.032"xsd:string
http://purl.uniprot.org/citations/20346955http://purl.uniprot.org/core/author"Ma K."xsd:string
http://purl.uniprot.org/citations/20346955http://purl.uniprot.org/core/author"Ma K."xsd:string
http://purl.uniprot.org/citations/20346955http://purl.uniprot.org/core/author"Wang K."xsd:string
http://purl.uniprot.org/citations/20346955http://purl.uniprot.org/core/author"Wang K."xsd:string
http://purl.uniprot.org/citations/20346955http://purl.uniprot.org/core/author"Qadota H."xsd:string
http://purl.uniprot.org/citations/20346955http://purl.uniprot.org/core/author"Qadota H."xsd:string
http://purl.uniprot.org/citations/20346955http://purl.uniprot.org/core/author"Benian G.M."xsd:string
http://purl.uniprot.org/citations/20346955http://purl.uniprot.org/core/author"Benian G.M."xsd:string
http://purl.uniprot.org/citations/20346955http://purl.uniprot.org/core/author"Flaherty D.B."xsd:string
http://purl.uniprot.org/citations/20346955http://purl.uniprot.org/core/author"Flaherty D.B."xsd:string
http://purl.uniprot.org/citations/20346955http://purl.uniprot.org/core/author"Forbes J.G."xsd:string
http://purl.uniprot.org/citations/20346955http://purl.uniprot.org/core/author"Forbes J.G."xsd:string
http://purl.uniprot.org/citations/20346955http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/20346955http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/20346955http://purl.uniprot.org/core/name"J. Mol. Biol."xsd:string
http://purl.uniprot.org/citations/20346955http://purl.uniprot.org/core/name"J. Mol. Biol."xsd:string
http://purl.uniprot.org/citations/20346955http://purl.uniprot.org/core/pages"672-689"xsd:string
http://purl.uniprot.org/citations/20346955http://purl.uniprot.org/core/pages"672-689"xsd:string
http://purl.uniprot.org/citations/20346955http://purl.uniprot.org/core/title"Extensive and modular intrinsically disordered segments in C. elegans TTN-1 and implications in filament binding, elasticity and oblique striation."xsd:string
http://purl.uniprot.org/citations/20346955http://purl.uniprot.org/core/title"Extensive and modular intrinsically disordered segments in C. elegans TTN-1 and implications in filament binding, elasticity and oblique striation."xsd:string
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