| http://purl.uniprot.org/citations/20347421 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20347421 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20347421 | http://www.w3.org/2000/01/rdf-schema#comment | "We describe a mechanistic model of polyubiquitination by the SCF(beta TrCP2) E3 ubiquitin (Ub) ligase using human I kappaB alpha as a substrate. Biochemical reconstitution experiments revealed that the polyubiquitination of I kappaB alpha began with the action of the UbcH5 E2 Ub-conjugating enzyme, transferring a single Ub to I kappaB alpha K21/K22 rapidly and efficiently. Subsequently, the Cdc34 E2 functioned in the formation of polyubiquitin chains. It was determined that a Ub fused at I kappaB alpha K21 acts as a receptor, directing Cdc34 for rapid and efficient K48-linked Ub chain synthesis that depends on SCF(beta TrCP2) and the substrate's N terminus. The I kappaB alpha-linked fusion Ub appears to mediate direct contacts with Cdc34 and the SCF's RING subcomplex. Taken together, these results suggest a role for the multifaceted interactions between the I kappaB alpha K21/K22-linked receptor Ub, the SCF's RING complex, and Cdc34 approximately S approximately Ub in establishing the optimal orientation of the receptor Ub to drive conjugation."xsd:string |
| http://purl.uniprot.org/citations/20347421 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.molcel.2010.02.025"xsd:string |
| http://purl.uniprot.org/citations/20347421 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.molcel.2010.02.025"xsd:string |
| http://purl.uniprot.org/citations/20347421 | http://purl.uniprot.org/core/author | "Wu K."xsd:string |
| http://purl.uniprot.org/citations/20347421 | http://purl.uniprot.org/core/author | "Wu K."xsd:string |
| http://purl.uniprot.org/citations/20347421 | http://purl.uniprot.org/core/author | "Pan Z.Q."xsd:string |
| http://purl.uniprot.org/citations/20347421 | http://purl.uniprot.org/core/author | "Pan Z.Q."xsd:string |
| http://purl.uniprot.org/citations/20347421 | http://purl.uniprot.org/core/author | "Kovacev J."xsd:string |
| http://purl.uniprot.org/citations/20347421 | http://purl.uniprot.org/core/author | "Kovacev J."xsd:string |
| http://purl.uniprot.org/citations/20347421 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20347421 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20347421 | http://purl.uniprot.org/core/name | "Mol. Cell"xsd:string |
| http://purl.uniprot.org/citations/20347421 | http://purl.uniprot.org/core/name | "Mol. Cell"xsd:string |
| http://purl.uniprot.org/citations/20347421 | http://purl.uniprot.org/core/pages | "784-796"xsd:string |
| http://purl.uniprot.org/citations/20347421 | http://purl.uniprot.org/core/pages | "784-796"xsd:string |
| http://purl.uniprot.org/citations/20347421 | http://purl.uniprot.org/core/title | "Priming and extending: a UbcH5/Cdc34 E2 handoff mechanism for polyubiquitination on a SCF substrate."xsd:string |
| http://purl.uniprot.org/citations/20347421 | http://purl.uniprot.org/core/title | "Priming and extending: a UbcH5/Cdc34 E2 handoff mechanism for polyubiquitination on a SCF substrate."xsd:string |
| http://purl.uniprot.org/citations/20347421 | http://purl.uniprot.org/core/volume | "37"xsd:string |
| http://purl.uniprot.org/citations/20347421 | http://purl.uniprot.org/core/volume | "37"xsd:string |
| http://purl.uniprot.org/citations/20347421 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20347421 |
| http://purl.uniprot.org/citations/20347421 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20347421 |
| http://purl.uniprot.org/citations/20347421 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20347421 |
| http://purl.uniprot.org/citations/20347421 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20347421 |