| http://purl.uniprot.org/citations/20367574 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20367574 | http://www.w3.org/2000/01/rdf-schema#comment | "Platelet glycoprotein (GP)Ib-IX-V, which binds von Willebrand factor (VWF), and GPVI, which binds collagen, form an adhesion-signaling complex on platelets and mediate platelet adhesion in flowing blood. Platelet activation following engagement of GPIb-IX-V/GPVI by VWF/collagen is critical for initiation and development of a protective thrombus across a site of damaged or exposed endothelium. We examined platelet aggregation and signaling following selective engagement of platelet GPIbalpha (the major ligand-binding subunit of GPIb-IX-V) by a multivalent surface-expressed GPIbalpha-binding VWF-A1 domain on COS-7 cells. COS-7 cells expressing the VWF-A1 domain containing an R543W mutation (a gain-of-function mutation found in Type 2B von Willebrand's Disease) were used as a selective agonist for GPIb-IX-V. When incubated in a cell-to-platelet ratio of up to 1 : 1200, VWF-A1/R543W cells caused rapid, spontaneous aggregation of washed platelets that was GPIbalpha- and alpha(IIb)beta(3)-dependent (blocked by inhibitory anti-VWF-A1, anti-GPIbalpha and anti-alpha(IIb)beta(3) antibodies). Platelet aggregation was also sensitive to inhibitors of Src, phosphoinositide 3-kinase (PI3-kinase) or Syk, confirming a role for these proteins in GPIbalpha-mediated signal transduction. Platelet tyrosine phosphorylation patterns and specific tyrosine phosphorylation of Syk after GPIbalpha engagement by VWF-A1/R543W was comparable to that induced by engagement of GPVI by collagen or collagen-related peptide (CRP). These data indicate signaling events triggered by specific ligation of GPIbalpha can lead to robust platelet activation and help define GPIb-IX-V as both an adhesion and signaling receptor on platelets."xsd:string |
| http://purl.uniprot.org/citations/20367574 | http://purl.org/dc/terms/identifier | "doi:10.3109/09537101003695339"xsd:string |
| http://purl.uniprot.org/citations/20367574 | http://purl.uniprot.org/core/author | "Berndt M.C."xsd:string |
| http://purl.uniprot.org/citations/20367574 | http://purl.uniprot.org/core/author | "Shen Y."xsd:string |
| http://purl.uniprot.org/citations/20367574 | http://purl.uniprot.org/core/author | "Andrews R.K."xsd:string |
| http://purl.uniprot.org/citations/20367574 | http://purl.uniprot.org/core/author | "Gardiner E.E."xsd:string |
| http://purl.uniprot.org/citations/20367574 | http://purl.uniprot.org/core/author | "Moore L.A."xsd:string |
| http://purl.uniprot.org/citations/20367574 | http://purl.uniprot.org/core/author | "Arthur J.F."xsd:string |
| http://purl.uniprot.org/citations/20367574 | http://purl.uniprot.org/core/author | "Karunakaran D."xsd:string |
| http://purl.uniprot.org/citations/20367574 | http://purl.uniprot.org/core/author | "Am Esch J.S."xsd:string |
| http://purl.uniprot.org/citations/20367574 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20367574 | http://purl.uniprot.org/core/name | "Platelets"xsd:string |
| http://purl.uniprot.org/citations/20367574 | http://purl.uniprot.org/core/pages | "244-252"xsd:string |
| http://purl.uniprot.org/citations/20367574 | http://purl.uniprot.org/core/title | "GPIbalpha-selective activation of platelets induces platelet signaling events comparable to GPVI activation events."xsd:string |
| http://purl.uniprot.org/citations/20367574 | http://purl.uniprot.org/core/volume | "21"xsd:string |
| http://purl.uniprot.org/citations/20367574 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20367574 |
| http://purl.uniprot.org/citations/20367574 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20367574 |
| http://purl.uniprot.org/uniprot/#_A0A193AU54-mappedCitation-20367574 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20367574 |
| http://purl.uniprot.org/uniprot/#_A0A193AUC0-mappedCitation-20367574 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20367574 |
| http://purl.uniprot.org/uniprot/#_A0A193AUJ5-mappedCitation-20367574 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20367574 |
| http://purl.uniprot.org/uniprot/#_A0A193AUV8-mappedCitation-20367574 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20367574 |
| http://purl.uniprot.org/uniprot/#_E0D851-mappedCitation-20367574 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20367574 |
| http://purl.uniprot.org/uniprot/#_E0D852-mappedCitation-20367574 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20367574 |
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