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http://purl.uniprot.org/citations/20369461http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20369461http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To investigate the effect of valproic acid (VPA) on MICA gene expression in human lung carcinoma cell lines: A549, SP-CA-1, NCIH446.

Methods

Different concentration of VPA were applied to treat A549, SP-CA-1, NCIH446 cells respectively and the untreated cells as the control. The effect of the VPA on the cells were observed. Reverse Transcriptase PCR and Immune-Fluorescence staining were used to detect the changes of mRNA, protein level of MICA gene in the three cell lines treated with 1.50 mmol/L and 3.00 mmol/L VPA for 4 days under the concentration of VPA from 0.75 to 12.0 mmol/L. The viability of lung cancer cells after NK effect in the VPA(+) and VPA(-) treatment were evaluated with LDH method.

Results

The inhibition effects were observed when the VPA concentration was 6.0 mmol/L above compared to control group, VPA induced the increasing expression of MICA and in turn enhanced the NK killing effect on the cells in a concentration-depended pattern.

Conclusion

VPA induced MICA expression, NK killing effect in the VPA(+) treatment lung cancer cells was significantly stronger than that of VPA(-) condition, which implied a new mechanism of anticancer method and could be a new approach to cancer therapy."xsd:string
http://purl.uniprot.org/citations/20369461http://purl.uniprot.org/core/author"Luo Y."xsd:string
http://purl.uniprot.org/citations/20369461http://purl.uniprot.org/core/author"Zhou J."xsd:string
http://purl.uniprot.org/citations/20369461http://purl.uniprot.org/core/author"Wang W."xsd:string
http://purl.uniprot.org/citations/20369461http://purl.uniprot.org/core/author"Yuan W."xsd:string
http://purl.uniprot.org/citations/20369461http://purl.uniprot.org/core/author"Long D."xsd:string
http://purl.uniprot.org/citations/20369461http://purl.uniprot.org/core/author"Li X.J."xsd:string
http://purl.uniprot.org/citations/20369461http://purl.uniprot.org/core/author"Fang B."xsd:string
http://purl.uniprot.org/citations/20369461http://purl.uniprot.org/core/author"Zhang Z.G."xsd:string
http://purl.uniprot.org/citations/20369461http://purl.uniprot.org/core/author"Rui J."xsd:string
http://purl.uniprot.org/citations/20369461http://purl.uniprot.org/core/author"Chou T."xsd:string
http://purl.uniprot.org/citations/20369461http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/20369461http://purl.uniprot.org/core/name"Sichuan Da Xue Xue Bao Yi Xue Ban"xsd:string
http://purl.uniprot.org/citations/20369461http://purl.uniprot.org/core/pages"10-14"xsd:string
http://purl.uniprot.org/citations/20369461http://purl.uniprot.org/core/title"[Valproic acid induce the expression of MICA antigen in human lung cancer cells and promote the NK killing effects on the cells]."xsd:string
http://purl.uniprot.org/citations/20369461http://purl.uniprot.org/core/volume"41"xsd:string
http://purl.uniprot.org/citations/20369461http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/20369461
http://purl.uniprot.org/citations/20369461http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/20369461
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http://purl.uniprot.org/uniprot/#_A0A0M3LB98-mappedCitation-20369461http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/20369461
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