Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/20372968http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20372968http://www.w3.org/2000/01/rdf-schema#comment"

Objective

This study investigates the effects of dipyrithione (PTS2) on the expression of IP-10/CXCL10, which has been observed in a wide variety of chronic inflammatory disorders and autoimmune conditions.

Methods

RAW264.7 cells (a murine macrophage-like cell line) were cultured in the absence or in the presence of PTS2 (3-10 microM) together with or without IFN-gamma (10 ng/ml). IP-10/CXCL10 expression was measured by specific enzyme-amplified immunoassays and reverse transcriptase-PCR (RT-PCR). Phosphorylation of JAK1, JAK2 and STAT1 were detected by Western blot analysis.

Results

We found that PTS2 inhibited IFN-gamma-induced up-regulation of IP-10/CXCL10 protein level in a dose- and time-dependent manner in RAW264.7 cells. RT-PCR experiments showed that PTS2 suppressed IFN-gamma-induced IP-10/CXCL10 expression at mRNA levels. Mechanistically, PTS2 prevented phosphorylation of JAK1, JAK2 and STAT1, but did not interfere with the p38 pathway. Furthermore, the inhibitory effect of PTS2 on IP-10/CXCL10 up-regulation was slightly stronger than JAK2 inhibitor AG490.

Conclusion

PTS2 inhibits IFN-gamma-induced IP-10/CXCL10 expression in RAW264.7 cells by targeting the JAK/STAT1 signaling pathway, suggesting that PTS2 could exert anti-inflammatory effects through attenuating the formation of chemokine IP-10/CXCL10."xsd:string
http://purl.uniprot.org/citations/20372968http://purl.org/dc/terms/identifier"doi:10.1007/s00011-010-0192-6"xsd:string
http://purl.uniprot.org/citations/20372968http://purl.uniprot.org/core/author"Han C."xsd:string
http://purl.uniprot.org/citations/20372968http://purl.uniprot.org/core/author"Huang H."xsd:string
http://purl.uniprot.org/citations/20372968http://purl.uniprot.org/core/author"Liu Z."xsd:string
http://purl.uniprot.org/citations/20372968http://purl.uniprot.org/core/author"Luo L."xsd:string
http://purl.uniprot.org/citations/20372968http://purl.uniprot.org/core/author"Yin Z."xsd:string
http://purl.uniprot.org/citations/20372968http://purl.uniprot.org/core/author"Fu J."xsd:string
http://purl.uniprot.org/citations/20372968http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/20372968http://purl.uniprot.org/core/name"Inflamm Res"xsd:string
http://purl.uniprot.org/citations/20372968http://purl.uniprot.org/core/pages"809-816"xsd:string
http://purl.uniprot.org/citations/20372968http://purl.uniprot.org/core/title"Dipyrithione inhibits IFN-gamma-induced JAK/STAT1 signaling pathway activation and IP-10/CXCL10 expression in RAW264.7 cells."xsd:string
http://purl.uniprot.org/citations/20372968http://purl.uniprot.org/core/volume"59"xsd:string
http://purl.uniprot.org/citations/20372968http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/20372968
http://purl.uniprot.org/citations/20372968http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/20372968
http://purl.uniprot.org/uniprot/#_A0A087WSP5-mappedCitation-20372968http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/20372968
http://purl.uniprot.org/uniprot/#_B1ASP2-mappedCitation-20372968http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/20372968
http://purl.uniprot.org/uniprot/#_P42225-mappedCitation-20372968http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/20372968
http://purl.uniprot.org/uniprot/#_P52332-mappedCitation-20372968http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/20372968
http://purl.uniprot.org/uniprot/#_Q3TI10-mappedCitation-20372968http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/20372968
http://purl.uniprot.org/uniprot/#_Q62120-mappedCitation-20372968http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/20372968
http://purl.uniprot.org/uniprot/#_Q3U8P8-mappedCitation-20372968http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/20372968
http://purl.uniprot.org/uniprot/#_Q3V3D6-mappedCitation-20372968http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/20372968
http://purl.uniprot.org/uniprot/#_Q3U6X5-mappedCitation-20372968http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/20372968
▶