| http://purl.uniprot.org/citations/20377743 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20377743 | http://www.w3.org/2000/01/rdf-schema#comment | "Gr3 is reported to play an important role in defense against viral infection. Although it is known that Gr3 is synthesized as a proenzyme and activated in the cytotoxic granules of NK cells and CTL, the activation mechanism is not clearly understood. In an attempt to analyze the activation mechanism of human Gr3, a recombinant pro-Gr3 was expressed in the periplasm of E. coli and purified to homogeneity. On SDS-PAGE the recombinant pro-Gr3 showed a slightly higher molecular weight than the enzymatically active Gr3, because the former possesses a small propeptide at its N-terminal. The recombinant pro-Gr3 was enzymatically inactive. It could be activated by treatment with cathepsin C, which concomitantly decreased the molecular weight to that of active Gr3. The proteolytic reaction of cathepsin C did not continue after one dipeptide had been removed, indicating that the recombinant pro-Gr3 had the native conformation without any refolding process. The recombinant pro-Gr3 would be a valuable tool for analyzing the activation mechanism and exploring other activating enzymes besides cathepsin C."xsd:string |
| http://purl.uniprot.org/citations/20377743 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1348-0421.2009.00187.x"xsd:string |
| http://purl.uniprot.org/citations/20377743 | http://purl.uniprot.org/core/author | "Inagaki H."xsd:string |
| http://purl.uniprot.org/citations/20377743 | http://purl.uniprot.org/core/author | "Hirata Y."xsd:string |
| http://purl.uniprot.org/citations/20377743 | http://purl.uniprot.org/core/author | "Kawada T."xsd:string |
| http://purl.uniprot.org/citations/20377743 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20377743 | http://purl.uniprot.org/core/name | "Microbiol Immunol"xsd:string |
| http://purl.uniprot.org/citations/20377743 | http://purl.uniprot.org/core/pages | "98-104"xsd:string |
| http://purl.uniprot.org/citations/20377743 | http://purl.uniprot.org/core/title | "Recombinant human progranzyme 3 expressed in Escherichia coli for analysis of its activation mechanism."xsd:string |
| http://purl.uniprot.org/citations/20377743 | http://purl.uniprot.org/core/volume | "54"xsd:string |
| http://purl.uniprot.org/citations/20377743 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20377743 |
| http://purl.uniprot.org/citations/20377743 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20377743 |
| http://purl.uniprot.org/uniprot/#_P49863-mappedCitation-20377743 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20377743 |
| http://purl.uniprot.org/uniprot/P49863 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20377743 |