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http://purl.uniprot.org/citations/20379057http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20379057http://www.w3.org/2000/01/rdf-schema#comment"

Aim

Ezetimibe is known to target Niemann-Pick Type C1 Like1 (NPC1L1), a key protein in intestinal cholesterol absorption, and thus to decrease serum LDL-cholesterol (LDL-C) levels. The response of serum LDL-C levels to ezetimibe was reported to differe among NPC1L1 haplotypes.We analyzed NPC1L1 genotypes in Japanese and investigated differences in markers of cholesterol synthesis/absorption among the genotypes.

Methods

Blood samples were collected from 42 adult volunteers to measure markers of cholesterol synthesis (lathosterol) and absorption (sitosterol and campesterol) by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Based on a study by Hegele RA et al. in Canada, we selected three SNPs (1735 C>G, 25342 A>C and 27677 T>C (numbers relative to the transcription start site)) and analyzed them using PCR-RFLP.

Results

The frequencies of genotypes were as follows: 1735 C/G (46%)>C/C (35%)>G/G (19%), 25342 A/A (97%)>A/C (3%)>C/C (0%) and 27677 T/T (97%)>T/C (3%)>C/C (0%). Serum campesterol levels were significantly higher in the 1735 G/G group than 1735 C/G+C/C group, but lathosterol levels showed no significant differences between the genotypes.

Conclusion

Our results revealed differences in the frequency of the NPC1L1 polymorphism between Japanese and Canadians. In Japanese, the 1735 G/G group showed enhanced cholesterol absorption from the intestine, as compared to the 1735 C/G+C/C group."xsd:string
http://purl.uniprot.org/citations/20379057http://purl.org/dc/terms/identifier"doi:10.5551/jat.2451"xsd:string
http://purl.uniprot.org/citations/20379057http://purl.uniprot.org/core/author"Ishikawa T."xsd:string
http://purl.uniprot.org/citations/20379057http://purl.uniprot.org/core/author"Kusano J."xsd:string
http://purl.uniprot.org/citations/20379057http://purl.uniprot.org/core/author"Kinoshita M."xsd:string
http://purl.uniprot.org/citations/20379057http://purl.uniprot.org/core/author"Matsuzaki Y."xsd:string
http://purl.uniprot.org/citations/20379057http://purl.uniprot.org/core/author"Tsukamoto K."xsd:string
http://purl.uniprot.org/citations/20379057http://purl.uniprot.org/core/author"Maeda T."xsd:string
http://purl.uniprot.org/citations/20379057http://purl.uniprot.org/core/author"Teramoto T."xsd:string
http://purl.uniprot.org/citations/20379057http://purl.uniprot.org/core/author"Honda A."xsd:string
http://purl.uniprot.org/citations/20379057http://purl.uniprot.org/core/author"Takeoka Y."xsd:string
http://purl.uniprot.org/citations/20379057http://purl.uniprot.org/core/author"Yasuda D."xsd:string
http://purl.uniprot.org/citations/20379057http://purl.uniprot.org/core/author"Mashimo Y."xsd:string
http://purl.uniprot.org/citations/20379057http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/20379057http://purl.uniprot.org/core/name"J Atheroscler Thromb"xsd:string
http://purl.uniprot.org/citations/20379057http://purl.uniprot.org/core/pages"356-360"xsd:string
http://purl.uniprot.org/citations/20379057http://purl.uniprot.org/core/title"A SNP of NPC1L1 affects cholesterol absorption in Japanese."xsd:string
http://purl.uniprot.org/citations/20379057http://purl.uniprot.org/core/volume"17"xsd:string
http://purl.uniprot.org/citations/20379057http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/20379057
http://purl.uniprot.org/citations/20379057http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/20379057
http://purl.uniprot.org/uniprot/#_A0A0C4DGG6-mappedCitation-20379057http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/20379057
http://purl.uniprot.org/uniprot/#_A0A0C4DFX6-mappedCitation-20379057http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/20379057
http://purl.uniprot.org/uniprot/#_Q9UHC9-mappedCitation-20379057http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/20379057
http://purl.uniprot.org/uniprot/Q9UHC9http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/20379057
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