| http://purl.uniprot.org/citations/20406883 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20406883 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20406883 | http://www.w3.org/2000/01/rdf-schema#comment | "Dictyostelium and human MidA are homologous proteins that belong to a family of proteins of unknown function called DUF185. Using yeast two-hybrid screening and pull-down experiments, we showed that both proteins interact with the mitochondrial complex I subunit NDUFS2. Consistent with this, Dictyostelium cells lacking MidA showed a specific defect in complex I activity, and knockdown of human MidA in HEK293T cells resulted in reduced levels of assembled complex I. These results indicate a role for MidA in complex I assembly or stability. A structural bioinformatics analysis suggested the presence of a methyltransferase domain; this was further supported by site-directed mutagenesis of specific residues from the putative catalytic site. Interestingly, this complex I deficiency in a Dictyostelium midA(-) mutant causes a complex phenotypic outcome, which includes phototaxis and thermotaxis defects. We found that these aspects of the phenotype are mediated by a chronic activation of AMPK, revealing a possible role of AMPK signaling in complex I cytopathology."xsd:string |
| http://purl.uniprot.org/citations/20406883 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.066076"xsd:string |
| http://purl.uniprot.org/citations/20406883 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.066076"xsd:string |
| http://purl.uniprot.org/citations/20406883 | http://purl.uniprot.org/core/author | "Calvo-Garrido J."xsd:string |
| http://purl.uniprot.org/citations/20406883 | http://purl.uniprot.org/core/author | "Calvo-Garrido J."xsd:string |
| http://purl.uniprot.org/citations/20406883 | http://purl.uniprot.org/core/author | "Carilla-Latorre S."xsd:string |
| http://purl.uniprot.org/citations/20406883 | http://purl.uniprot.org/core/author | "Carilla-Latorre S."xsd:string |
| http://purl.uniprot.org/citations/20406883 | http://purl.uniprot.org/core/author | "Escalante R."xsd:string |
| http://purl.uniprot.org/citations/20406883 | http://purl.uniprot.org/core/author | "Escalante R."xsd:string |
| http://purl.uniprot.org/citations/20406883 | http://purl.uniprot.org/core/author | "Fisher P.R."xsd:string |
| http://purl.uniprot.org/citations/20406883 | http://purl.uniprot.org/core/author | "Fisher P.R."xsd:string |
| http://purl.uniprot.org/citations/20406883 | http://purl.uniprot.org/core/author | "Valencia A."xsd:string |
| http://purl.uniprot.org/citations/20406883 | http://purl.uniprot.org/core/author | "Valencia A."xsd:string |
| http://purl.uniprot.org/citations/20406883 | http://purl.uniprot.org/core/author | "Annesley S.J."xsd:string |
| http://purl.uniprot.org/citations/20406883 | http://purl.uniprot.org/core/author | "Annesley S.J."xsd:string |
| http://purl.uniprot.org/citations/20406883 | http://purl.uniprot.org/core/author | "Accari S.L."xsd:string |
| http://purl.uniprot.org/citations/20406883 | http://purl.uniprot.org/core/author | "Accari S.L."xsd:string |
| http://purl.uniprot.org/citations/20406883 | http://purl.uniprot.org/core/author | "Gallardo M.E."xsd:string |
| http://purl.uniprot.org/citations/20406883 | http://purl.uniprot.org/core/author | "Gallardo M.E."xsd:string |
| http://purl.uniprot.org/citations/20406883 | http://purl.uniprot.org/core/author | "Garesse R."xsd:string |
| http://purl.uniprot.org/citations/20406883 | http://purl.uniprot.org/core/author | "Garesse R."xsd:string |
| http://purl.uniprot.org/citations/20406883 | http://purl.uniprot.org/core/author | "Grana O."xsd:string |
| http://purl.uniprot.org/citations/20406883 | http://purl.uniprot.org/core/author | "Grana O."xsd:string |